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Evaluation of commercial polyclonal- and monoclonal-antibody-based immunohistochemical tests for 2 genotypes of Porcine circovirus type 2 and comparison with in-situ hybridization assays

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dc.contributor.authorSeo, Hwi Won-
dc.contributor.authorHan, Kiwon-
dc.contributor.authorOh, Yeonsu-
dc.contributor.authorKang, Ikjae-
dc.contributor.authorPark, Changhoon-
dc.contributor.authorJoo, Hye Eun-
dc.contributor.authorKim, Sung-Hoon-
dc.contributor.authorLee, Bog-Hieu-
dc.contributor.authorChae, Chanhee-
dc.date.available2019-03-08T21:41:52Z-
dc.date.issued2014-07-
dc.identifier.issn0830-9000-
dc.identifier.urihttps://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12121-
dc.description.abstractThe objective of the present study was to evaluate polyclonal-and monoclonal-antibody-based immunohistochemical (IHC) tests for the detection of 2 genotypes of Porcine circovirus type 2 (PCV2), a and b, in formalin-fixed, paraffin-embedded lymph-node tissue from pigs with experimental or natural postweaning multisystemic wasting syndrome and to compare the IHC results with those of in-situ hybridization (ISH) assays. The ISH assays proved more sensitive than the IHC tests for the detection of PCV2a and PCV2b. According to these findings, polyclonal-antibody-based IHC testing is the most practical routine diagnostic method for the detection of PCV2 regardless of genotype because IHC testing is less technically complex than ISH testing. However, ISH assays are useful to differentiate between PCV2a and PCV2b in surveillance programs for the monitoring of PCV2 in swine herds.-
dc.format.extent4-
dc.language영어-
dc.language.isoENG-
dc.publisherCANADIAN VET MED ASSOC-
dc.titleEvaluation of commercial polyclonal- and monoclonal-antibody-based immunohistochemical tests for 2 genotypes of Porcine circovirus type 2 and comparison with in-situ hybridization assays-
dc.typeArticle-
dc.identifier.bibliographicCitationCANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, v.78, no.3, pp 233 - 236-
dc.description.isOpenAccessN-
dc.identifier.wosid000346497000011-
dc.identifier.scopusid2-s2.0-84903542977-
dc.citation.endPage236-
dc.citation.number3-
dc.citation.startPage233-
dc.citation.titleCANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE-
dc.citation.volume78-
dc.type.docTypeArticle-
dc.publisher.location캐나다-
dc.subject.keywordPlusMULTISYSTEMIC WASTING SYNDROME-
dc.subject.keywordPlusPARAFFIN-EMBEDDED TISSUES-
dc.subject.keywordPlusPIGS-
dc.subject.keywordPlusREPLICATION-
dc.subject.keywordPlusPORCINE-CIRCOVIRUS-2-
dc.subject.keywordPlusVACCINATION-
dc.subject.keywordPlusPARVOVIRUS-
dc.subject.keywordPlusINFECTION-
dc.subject.keywordPlusVIRUS-
dc.subject.keywordPlusCELLS-
dc.relation.journalResearchAreaVeterinary Sciences-
dc.relation.journalWebOfScienceCategoryVeterinary Sciences-
dc.description.journalRegisteredClasssci-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
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