Glycoengineering of Interferon-beta 1a Improves Its Biophysical and Pharmacokinetic Properties
DC Field | Value | Language |
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dc.contributor.author | Song, Kyoung | - |
dc.contributor.author | Yoon, In-Soo | - |
dc.contributor.author | Kim, Nam Ah | - |
dc.contributor.author | Kim, Dong-Hwan | - |
dc.contributor.author | Lee, Jongmin | - |
dc.contributor.author | Lee, Hee Jung | - |
dc.contributor.author | Lee, Saehyung | - |
dc.contributor.author | Choi, Sunghyun | - |
dc.contributor.author | Choi, Min-Koo | - |
dc.contributor.author | Kim, Ha Hyung | - |
dc.contributor.author | Jeong, Seong Hoon | - |
dc.contributor.author | Son, Woo Sung | - |
dc.contributor.author | Kim, Dae-Duk | - |
dc.contributor.author | Shin, Young Kee | - |
dc.date.available | 2019-03-08T21:57:34Z | - |
dc.date.issued | 2014-05-23 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12221 | - |
dc.description.abstract | The purpose of this study was to develop a biobetter version of recombinant human interferon-beta 1a (rhIFN-beta 1a) to improve its biophysical properties, such as aggregation, production and stability, and pharmacokinetic properties without jeopardizing its activity. To achieve this, we introduced additional glycosylation into rhIFN-beta 1a via site-directed mutagenesis. Glycoengineering of rhIFN-beta 1a resulted in a new molecular entity, termed R27T, which was defined as a rhIFN-beta mutein with two N-glycosylation sites at 80th (original site) and at an additional 25th amino acid due to a mutation of Thr for Arg at position 27th of rhIFN-beta 1a. Glycoengineering had no effect on rhIFN-beta ligand-receptor binding, as no loss of specific activity was observed. R27T showed improved stability and had a reduced propensity for aggregation and an increased half-life. Therefore, hyperglycosylated rhIFN-beta could be a biobetter version of rhIFN-beta 1a with a potential for use as a drug against multiple sclerosis. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Glycoengineering of Interferon-beta 1a Improves Its Biophysical and Pharmacokinetic Properties | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0096967 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.9, no.5 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000336839400007 | - |
dc.identifier.scopusid | 2-s2.0-84901414491 | - |
dc.citation.number | 5 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | MULTIPLE-SCLEROSIS | - |
dc.subject.keywordPlus | POLYETHYLENE-GLYCOL | - |
dc.subject.keywordPlus | PROTEIN AGGREGATION | - |
dc.subject.keywordPlus | RECEPTOR-BINDING | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | SITE | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | PEGYLATION | - |
dc.subject.keywordPlus | THERAPIES | - |
dc.subject.keywordPlus | STABILITY | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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