Functional Conservation of RNase III-like Enzymes: Studies on a Vibrio vulnificus Ortholog of Escherichia coli RNase III
DC Field | Value | Language |
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dc.contributor.author | Lee, Minho | - |
dc.contributor.author | Ahn, Sangmi | - |
dc.contributor.author | Lim, Boram | - |
dc.contributor.author | Lee, Dong-Ho | - |
dc.contributor.author | Lee, Kangseok | - |
dc.date.available | 2019-03-08T21:59:59Z | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 0343-8651 | - |
dc.identifier.issn | 1432-0991 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12332 | - |
dc.description.abstract | Bacterial ribonuclease III (RNase III) belongs to the RNase III enzyme family, which plays a pivotal role in controlling mRNA stability and RNA processing in both prokaryotes and eukaryotes. In the Vibrio vulnificus genome, one open reading frame encodes a protein homologous to E. coli RNase III, designated Vv-RNase III, which has 77.9 % amino acid identity to E. coli RNase III. Here, we report that Vv-RNase III has the same cleavage specificity as E. coli RNase III in vivo and in vitro. Expressing Vv-RNase III in E. coli cells deleted for the RNase III gene (rnc) restored normal rRNA processing and, consequently, growth rates of these cells comparable to wild-type cells. In vitro cleavage assays further showed that Vv-RNase III has the same cleavage activity and specificity as E. coli RNase III on RNase III-targeted sequences of corA and mltD mRNA. Our findings suggest that RNase III-like proteins have conserved cleavage specificity across bacterial species. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Functional Conservation of RNase III-like Enzymes: Studies on a Vibrio vulnificus Ortholog of Escherichia coli RNase III | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00284-013-0492-5 | - |
dc.identifier.bibliographicCitation | CURRENT MICROBIOLOGY, v.68, no.4, pp 413 - 418 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000331006200001 | - |
dc.identifier.scopusid | 2-s2.0-84893976215 | - |
dc.citation.endPage | 418 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 413 | - |
dc.citation.title | CURRENT MICROBIOLOGY | - |
dc.citation.volume | 68 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | RIBONUCLEASE-III | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | ENDORIBONUCLEASE-III | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | RIBOSOMAL-RNA | - |
dc.subject.keywordPlus | MINI-III | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | MATURATION | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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