The Effect of DA-9701 on 5-Hydroxytryptamine-Induced Contraction of Feline Esophageal Smooth Muscle Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Kyung Hoon | - |
dc.contributor.author | Nam, Yoonjin | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Kim, In Kyeom | - |
dc.contributor.author | Sohn, Uy Dong | - |
dc.date.available | 2019-03-08T22:00:58Z | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12369 | - |
dc.description.abstract | Serotonin, or 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter found in blood platelets, the gastrointestinal (GI) tract, and the central nervous system (CNS) of animals and humans. The signaling pathways of 5-hydroxytryptamine (5-HT)-induced contractions in cat esophageal smooth muscle cell (ESMC)s have been identified, but the downstream components of the 5-HT signaling pathway remain unclear. DA-9701 is the standardized extract of the Pharbitis nil Choisy seed (Pharbitidis Semen, Convolvulaceae) and the root of Corydalis yahusuo W. T. Wang (Corydalis Tuber, Papaveraceae). DA-9701 is known to have strong gastroprokinetic effects and a good safety profile. In this study, we investigated the 5-HT signaling pathway at the G-protein level, and we explored the mechanisms by which DA-9701 induces smooth muscle contraction. Freshly isolated smooth muscle cells were harvested from the feline esophagus, and cells were permeabilized to measure their length. 5-HT produced esophageal smooth muscle contractions in a dose-dependent manner. Furthermore, 5-HT produced a relatively long-acting contraction. 5-HT binds to the 5-HT2, 5-HT3 and 5-HT4 receptors to induce smooth muscle contraction in feline ESMCs. These receptors, which are located in esophageal smooth muscle, are coupled to G(alpha q), G(alpha o) and G(alpha s). These G proteins activate PLC, which leads to Ca2+/calmodulin-dependent MLCK activation, resulting in MLC20 phosphorylation and cell contraction. Conversely, DA-9701 inhibits 5-HT-induced contraction by inhibiting MLC20 phosphorylation. | - |
dc.format.extent | 15 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | The Effect of DA-9701 on 5-Hydroxytryptamine-Induced Contraction of Feline Esophageal Smooth Muscle Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/molecules19045135 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.19, no.4, pp 5135 - 5149 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000336087800081 | - |
dc.identifier.scopusid | 2-s2.0-84899538014 | - |
dc.citation.endPage | 5149 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 5135 | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 19 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | 5-hydroxytryptamine | - |
dc.subject.keywordAuthor | feline esophageal smooth muscle | - |
dc.subject.keywordAuthor | DA-9701 | - |
dc.subject.keywordAuthor | Pharbitidis Semen | - |
dc.subject.keywordAuthor | Corydalis Tuber | - |
dc.subject.keywordAuthor | MLC20 | - |
dc.subject.keywordAuthor | phosphorylation | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | SEROTONIN RECEPTORS | - |
dc.subject.keywordPlus | ADENYLYL-CYCLASE | - |
dc.subject.keywordPlus | CAT ESOPHAGEAL | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | SPHINCTER | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.