Comparative Analysis of Flavonoids and Polar Metabolite Profiling of Tanno-Original and Tanno-High Rutin Buckwheat
DC Field | Value | Language |
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dc.contributor.author | Li, Xiaohua | - |
dc.contributor.author | Kim, Jae Kwang | - |
dc.contributor.author | Park, Soo-Yun | - |
dc.contributor.author | Zhao, Shicheng | - |
dc.contributor.author | Kim, Yeon Bok | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Park, Sang Un | - |
dc.date.available | 2019-03-08T22:01:32Z | - |
dc.date.issued | 2014-03 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.issn | 1520-5118 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12389 | - |
dc.description.abstract | Rutin is an important indicator for evaluating the quality of buckwheat. In this study, flavonoid biosynthesis was compared between two common cultivars (an original and a high-rutin line) of buckwheat, Fagopyrum esculentum Moench. Transcriptional levels of the main flavonoid biosynthetic genes were analyzed by real-time PCR, and main flavonoid metabolites were detected by high-performance liquid chromatography (HPLC); levels of gene expression varied among organs of the two cultivars. Significantly higher transcription levels of most flavonoid biosynthetic genes, except FeFLS1, were detected in stems of the high-rutin line than in stems of the original line. FeCH1 and FeFLS2 genes also showed higher expression levels in seeds of the high-rutin cultivar. In contrast, FePAL, FeC4H, Fe4CL1, FeCHS, FeF3H, FeF3'H, FeFLS2, and FeDFR were highly detected in the roots of the original line. The HPLC results indicated 1.73-, 1.62-, and 1.77-fold higher accumulation of rutin (the primary flavonoid compound) in leaves, stems, and mature seeds of the high-rutin cultivar (24.86, 1.46, and 1.36 mu g/mg, respectively) compared with the original cultivar (14.40, 0.90, and 0.77 mu g/mg, respectively). A total of 46 metabolites were identified from seeds by gas chromatography-time-of-flight mass spectrometry. The metabolite profiles were subjected to principal component analysis (PCA). PCA could clearly differentiate the original and high-rutin cultivars. Our results indicate that the high-rutin cultivar could be an excellent alternative for buckwheat culture, and we provide useful information for obtaining this cultivar. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Comparative Analysis of Flavonoids and Polar Metabolite Profiling of Tanno-Original and Tanno-High Rutin Buckwheat | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/jf4049534 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.62, no.12, pp 2701 - 2708 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000333551600030 | - |
dc.identifier.scopusid | 2-s2.0-84896925079 | - |
dc.citation.endPage | 2708 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2701 | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 62 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Fagopyrum esculentum | - |
dc.subject.keywordAuthor | flavonoid biosynthetic pathway | - |
dc.subject.keywordAuthor | gene expression | - |
dc.subject.keywordAuthor | rutin biosynthesis | - |
dc.subject.keywordPlus | FAGOPYRUM-ESCULENTUM MOENCH | - |
dc.subject.keywordPlus | LIGASE GENE FAMILY | - |
dc.subject.keywordPlus | TARTARY BUCKWHEAT | - |
dc.subject.keywordPlus | PHENOLIC-COMPOUNDS | - |
dc.subject.keywordPlus | DIFFERENTIAL EXPRESSION | - |
dc.subject.keywordPlus | HYPERLIPIDEMIC RATS | - |
dc.subject.keywordPlus | BIOSYNTHESIS GENES | - |
dc.subject.keywordPlus | TATARICUM GAERTN. | - |
dc.subject.keywordPlus | F-HOMOTROPICUM | - |
dc.subject.keywordPlus | BRAN EXTRACT | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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