Anti-carcinogenic actions of glycoprotein conjugated with isoflavones from submerged-liquid culture of Agaricus blazei mycelia through reciprocal expression of Bcl-2 and Bax proteins
DC Field | Value | Language |
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dc.contributor.author | 김영석 | - |
dc.contributor.author | 김보현 | - |
dc.contributor.author | 김곤섭 | - |
dc.contributor.author | 장정순 | - |
dc.contributor.author | 김소영 | - |
dc.contributor.author | 최병대 | - |
dc.contributor.author | 김정옥 | - |
dc.contributor.author | 하영래 | - |
dc.date.available | 2019-03-08T22:58:42Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 2508-1357 | - |
dc.identifier.issn | 2508-139X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12926 | - |
dc.description.abstract | Glycoproteins isolated from fruit bodies and mycelial cultures of mushrooms exhibit anti-carcinogenic actions in human cancer cells and animal tumor cells by induction of apoptosis. Here, we report that isoflavone-conjugated glycoproteins (designate Gluvone), exhibit strong anti- carcinogenic effects on human breast cancer MCF-7 cells by induction of apoptosis. Gluvone with 9.4 kDa of molecular weight was isolated from submerged-liquid culture of Agaricus blazei mycelia (ABM) in soy flake-containing liquid medium. MCF-7 cells were incubated with various amounts of Gluvone (0~250 μM) for a period of 6 days. Gluvone exhibited anti-proliferative actions in a dose-dependent manner and 62% growth inhibition at 200 μM for 4 days relative to control. Hoechst 33258 staining analysis revealed that Gluvone induced formation of apoptotic bodies. Gluvone was associated with down-regulation of anti-apoptotic Bcl-2 protein expression as well as up-regulation of pro- apoptotic Bax protein expression. Gluvone treatment induced proteolytic activation of caspase-9 and caspase-3 through cytochrome c release from mitochondria to cytosol as well as concomitant degradation of poly (ADP-ribose) polymerase (PARP). In addition, Gluvone induced activation of caspase-8. Taken all together, these results indicate that the anti- proliferative effect of Gluvone is associated with induction of apoptotic cell death through the mitochondrial dysfunction pathway mediated by enhancement of Bax protein expression and suppression of Bcl-2 protein expression. | - |
dc.format.extent | 7 | - |
dc.publisher | 충북대학교 동물의학연구소 | - |
dc.title | Anti-carcinogenic actions of glycoprotein conjugated with isoflavones from submerged-liquid culture of Agaricus blazei mycelia through reciprocal expression of Bcl-2 and Bax proteins | - |
dc.type | Article | - |
dc.identifier.doi | 10.12729/jbr.2014.15.4.200 | - |
dc.identifier.bibliographicCitation | Journal of Biomedical and Translational Research, v.15, no.4, pp 200 - 206 | - |
dc.identifier.kciid | ART001951990 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 206 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 200 | - |
dc.citation.title | Journal of Biomedical and Translational Research | - |
dc.citation.volume | 15 | - |
dc.subject.keywordAuthor | Agaricus blazei mycelium (ABM) | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | caspase 3 | - |
dc.subject.keywordAuthor | human breast cancer MCF-7 cells | - |
dc.subject.keywordAuthor | isoflavone-conjugated glycoproteins (Gluvone) | - |
dc.description.journalRegisteredClass | kciCandi | - |
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