Ethyl Acetate와 Methanol을 이용한 블루베리 추출물 대사체 분석
DC Field | Value | Language |
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dc.contributor.author | Jo, Young-Hee | - |
dc.contributor.author | Kim, Sugyeong | - |
dc.contributor.author | Kwon, Da-Ae | - |
dc.contributor.author | Lee, Hong Jin | - |
dc.contributor.author | Choi, Hyung-Kyoon | - |
dc.contributor.author | Auh, Joong-Hyuck | - |
dc.date.available | 2019-03-09T00:40:06Z | - |
dc.date.issued | 2014-03 | - |
dc.identifier.issn | 1226-3311 | - |
dc.identifier.issn | 2288-5978 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/13848 | - |
dc.description.abstract | 본 연구에서는 LC-MS/MS를 이용한 블루베리의 methanol과 ethyl acetate 추출 분획에 존재하는 대사체의 분석을 통해 효율적인 대사체 profiling의 가능성을 탐색하였다. LC-MS/MS에서 검출되는 대사체를 통계 처리한 결과, methanol 추출 분획에서는 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, delphinidin hexoside, delphinidin, petunidin hexoside와 같은 안토시아닌 계열의 화합물들이 존재하였고, ethyl acetate 분획에서는 chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosylluteolin, luteolin과 같은 플라보노이드 계열의 화합물이 검출되었다. 본 연구는 기존 연구와 달리 대사체학 기법을 이용한 블루베리 추출물 전체 대사물질의 profiling을 시도한 최초의 연구로서 블루베리에 함유된 유용 성분의 스크리닝등 향후 응용 연구에 유용한 기반으로 이용될 수 있을 것으로 기대된다. | - |
dc.description.abstract | Metabolite profiling of blueberry (cultivar Spartan) was performed by extraction using different solvents, methanol and ethyl acetate, through metabolomic analysis using LC-MS/MS. Unsupervised classification method (PCA) and supervised prediction model (OPLS-DA) provided good categorization of metabolites according to the extraction solvents. Metabolites of the anthocyanin family, including delphinidin hexoside, delphinidin, 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, and petunidin hexoside, were mainly detected in methanol fractions, whereas those of the flavonoid family, including chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosyl-luteolin, and luteolin were successfully prepared in the ethyl acetate fraction. Thus, metabolomic analysis of blueberry extracts allows for the simple profiling of whole and distinctive metabolites for future applications. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society of Food Science and Nutrition | - |
dc.title | Ethyl Acetate와 Methanol을 이용한 블루베리 추출물 대사체 분석 | - |
dc.title.alternative | Metabolomic analysis of ethyl acetate and methanol extracts of blueberry | - |
dc.type | Article | - |
dc.identifier.doi | 10.3746/jkfn.2014.43.3.419 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society of Food Science and Nutrition, v.43, no.3, pp 419 - 424 | - |
dc.identifier.kciid | ART001861009 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84929408082 | - |
dc.citation.endPage | 424 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 419 | - |
dc.citation.title | Journal of the Korean Society of Food Science and Nutrition | - |
dc.citation.volume | 43 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Blueberry | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordAuthor | Metabolites profiling | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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