Stability of virus-like particles of red-spotted grouper nervous necrosis virus in the aqueous state, and the vaccine potential of lyophilized particles
- Authors
- Nguyen Thi Lan; Kim, Hyoung Jin; Han, Hyun-Ja; Lee, Deok-Chan; Kang, Bo Kyu; Han, Sang Yoon; Moon, Hyoungjoon; Kim, Hong-Jin
- Issue Date
- Jan-2018
- Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Keywords
- Nervous necrosis virus; Virus-like particles; Freeze drying; Vaccine; Lyophilization
- Citation
- BIOLOGICALS, v.51, pp 25 - 31
- Pages
- 7
- Journal Title
- BIOLOGICALS
- Volume
- 51
- Start Page
- 25
- End Page
- 31
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1400
- DOI
- 10.1016/j.biologicals.2017.11.002
- ISSN
- 1045-1056
1095-8320
- Abstract
- Virus-like particles (VLPs) are multi protein complexes mimicking the structural properties of the native virus. The development of freeze-dried formulations of such complex protein structures remains a challenge. Red spotted grouper nervous necrosis virus (RGNNV) causes mass mortality in fish culture, and RGNNV VLPs have been suggested to be promising vaccine candidates. In the present study, the stability of RGNNV VLPs in the liquid state was investigated over a 4-week period, along with the influence of freeze-drying on VLP stability. RGNNV VLPs were completely degraded after one week at 37 degrees C followed by 3 weeks at ambient temperature, and they were partially degraded after 4 weeks at 4 degrees C. Therefore, the inherent stability of RGNNV VLP in an aqueous milieu is insufficient for long-term storage. When RGNNV VLPs were freeze-dried in the presence or absence of sugar stabilizers, sorbitol was found to improve VLP stability whereas mannitol reduced it. VLP preparations freeze-dried with sorbitol or without stabilizer were as immunogenic as control (non-freeze dried) VLPs, whereas VLPs freeze-dried in mannitol were less immunogenic. These results indicate that freeze-dried RGNNV VLPs have potential as vaccines.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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