Paenibacillus limicola sp nov., isolated from tidal flat sediment

Abstract

An aerobic, Gram-staining-variable, rod-shaped, endospore-forming and motile bacterial strain, designated CJ6(T), was isolated from a tidal flat on Ganghwa Island, South Korea. The isolate was characterized based on a polyphasic taxonomy approach. Strain CJ6(T) grew optimally on R2A agar media at 30 degrees C and pH 7. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ6(T) belonged to the genus Paenibacillus, displaying the highest sequence similarity to Paenibacillus vulneris CCUG 53270(T) (97.0 %) and clearly defined strain CJ6(T) as a novel species within the genus. The G+C content of the genomic DNA was 49.9 mol%. The major polar lipid contents of strain CJ6(T) were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified glycolipids. MK-7 was detected as the major respiratory quinone. The dominant fatty acid was anteiso-C-15:0. Analyses of phylogenetic, phenotypic, biochemical and chemotaxonomic characteristics indicated that strain CJ6(T) was distinguishable from its closely related type strains. Therefore, strain CJ6(T) represents a novel species in the genus Paenibacillus, for which name Paenibacillus limicola sp. nov. is proposed; the type strain is CJ6(T) (=KACC 19303(T) =JCM 32079(T)).