Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation
DC Field | Value | Language |
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dc.contributor.author | Cha, Young Joo | - |
dc.contributor.author | Yoo, Soo Jin | - |
dc.contributor.author | Sohn, Yong-Hak | - |
dc.contributor.author | Kim, Hyun Soo | - |
dc.date.available | 2019-03-09T01:00:12Z | - |
dc.date.issued | 2013-11 | - |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.issn | 1879-0984 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14155 | - |
dc.description.abstract | The performance of a recently developed real-time PCR system, the ExiStation HBV diagnostic system, for quantitation of hepatitis B virus (HBV) in human blood was evaluated. The detection limit, reproducibility, cross-reactivity, and interference were evaluated as measures of analytical performance. For the comparison study, 100 HBV-positive blood samples and 100 HBV-negative samples from Korean Blood Bank Serum were used, and the results of the ExiStation HBV system showed good correlation with those obtained using the Cobas TaqMan (r(2) = 0.9931) and Abbott real-time PCR systems (r(2) = 0.9894). The lower limit of detection was measured as 9.55 IU/mL using WHO standards and the dynamic range was linear from 6.68 to 6.68 x 10(9) IU/mL using cloned plasmids. The within-run coefficient of variation (CV) was 9.4%, 2.1%, and 1.1%, and the total CV was 11.8%, 3.6%, and 1.7% at a concentration of 1.92 log(10) IU/mL, 3.88 log(10) IU/mL, and 6.84 log(10) IU/mL, respectively. No cross-reactivity or interference was detected. The ExiStation HBV diagnostic system showed satisfactory analytical sensitivity, excellent reproducibility, no cross-reactivity, no interference, and high agreement with the Cobas TaqMan and Abbott real-time PCR systems, and is therefore a useful tool for the detection and monitoring of HBV infection. (C) 2013 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jviromet.2013.07.027 | - |
dc.identifier.bibliographicCitation | JOURNAL OF VIROLOGICAL METHODS, v.193, no.2, pp 492 - 497 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000325190400038 | - |
dc.identifier.scopusid | 2-s2.0-84882684823 | - |
dc.citation.endPage | 497 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 492 | - |
dc.citation.title | JOURNAL OF VIROLOGICAL METHODS | - |
dc.citation.volume | 193 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Hepatitis B virus | - |
dc.subject.keywordAuthor | Real-time PCR | - |
dc.subject.keywordAuthor | Quantitation | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | PLASMA | - |
dc.subject.keywordPlus | CYTOMEGALOVIRUS | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | ASSAY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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