Quercetin inhibits lipopolysaccharide-induced nitric oxide production in BV2 microglial cells by suppressing the NF-kappa B pathway and activating the Nrf2-dependent HO-1 pathway
DC Field | Value | Language |
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dc.contributor.author | Kang, Chang-Hee | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Kim, Gi-Young | - |
dc.date.available | 2019-03-09T01:00:53Z | - |
dc.date.issued | 2013-11 | - |
dc.identifier.issn | 1567-5769 | - |
dc.identifier.issn | 1878-1705 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14181 | - |
dc.description.abstract | Abnormal nitrosative stress-induced neuroinflammation is implicated in the pathogenesis of neurodegenerative diseases. Therefore, it has been thought that nitric oxide (NO) production is a good therapeutic target. In this sense, quercetin is a good chemopreventive component, because it has free radical-scavenging and anti-inflammatory activities. However, explicit mechanisms are not clear in the lipopolysaccharide (LPS)-stimulated BV2 microglial cell line. Here, we found that quercetin significantly suppressed LPS-induced NO production and inducible NO synthase (iNOS) expression. Notably, quercetin inhibited nuclear factor-kappa B (NF-kappa B) activation by inhibiting degradation of the inhibitor of kappa B alpha (I kappa B alpha) in LPS-stimulated BV2 microglial cells corresponding to the inhibitory effect of specific NF-kappa B inhibitors, namely proteasome inhibitor I (PSI) and MG132. Quercetin caused significant increases in the levels of heme oxgenase-1 (HO-1) mRNA and protein. Notably, treatment with an HO-1 inducer, cobalt protoporphyrin (CoPP), significantly diminished LPS-stimulated NO production. Additionally, quercetin induced the specific DNA-binding activity of nuclear factor-2-erythroid 2-related factor 2 (Nrf2), and siRNA-mediated knockdown of Nrf2 expression reduced the inhibitory effect of quercetin on LPS-stimulated NO production by inhibiting HO-1 expression, indicating that quercetin regulated NO production by inducing Nrf2-mediated HO-1 expression. Therefore, quercetin has the potential to decrease nitrosative stress by suppressing NF-kappa B activation and inducing Nrf2-mediated HO-1 expression. (C) 2013 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Quercetin inhibits lipopolysaccharide-induced nitric oxide production in BV2 microglial cells by suppressing the NF-kappa B pathway and activating the Nrf2-dependent HO-1 pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.intimp.2013.09.009 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL IMMUNOPHARMACOLOGY, v.17, no.3, pp 808 - 813 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000327280100044 | - |
dc.identifier.scopusid | 2-s2.0-84884921233 | - |
dc.citation.endPage | 813 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 808 | - |
dc.citation.title | INTERNATIONAL IMMUNOPHARMACOLOGY | - |
dc.citation.volume | 17 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Quercetin | - |
dc.subject.keywordAuthor | Nitric oxide | - |
dc.subject.keywordAuthor | Nitric oxide synthase | - |
dc.subject.keywordAuthor | Nuclear factor-kappa B | - |
dc.subject.keywordAuthor | Heme oxgenase-1 | - |
dc.subject.keywordAuthor | Nuclear factor-2-erythroid 2-related factor 2 | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 INDUCTION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | EX-VIVO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | INOS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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