Interleukin-5 enhances the migration and invasion of bladder cancer cells via ERK1/2-mediated MMP-9/NF-kappa B/AP-1 pathway: Involvement of the p21WAF1 expression
DC Field | Value | Language |
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dc.contributor.author | Lee, Eo-Jin | - |
dc.contributor.author | Lee, Se-Jung | - |
dc.contributor.author | Kim, Sangtae | - |
dc.contributor.author | Cho, Seok-Cheol | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.date.available | 2019-03-09T01:02:16Z | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.issn | 1873-3913 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14236 | - |
dc.description.abstract | Inflammatory cytokines may be a critical component of epithelial cancer progression. We examined the role of interleukin (IL)-5 in the migration of bladder cancer cells. The expression of IL-5 and its receptor IL-5R alpha was enhanced in patients with muscle invasive bladder cancers (MIBC), and then it was detected in bladder cancer cell lines 5637 and T-24. IL-5 increased migration and MMP-9 expression via activation of transcription factors NF-kappa B and AP-1, and induced activation of ERK1/2 and Jak-Stat signaling in both cells. Treatment with ERK1/2 inhibitor U0126 significantly inhibited induction of migration, MMP-9 expression, and activation of NF-kappa B and AP-1 in IL-5-treated cells. However, none of the Jak inhibitors affected the IL-5-induced migration of bladder cancer cells. Moreover, gene knockdown for IL-5R alpha, using siRNA transfection, suppressed migration, ERK1/2 activation, MMP-9 expression, as well as the binding activation of NF-kappa B and AP-1 in IL-5-treated bladder cancer cells. Similar results were observed in beta c siRNA (si-beta c) transfected cells. Unexpectedly, IL-5 treatment resulted in significant induction of p21WAF1 in both cell lines. The p21WAF1-specific small interfering RNA inhibited IL-5-induced cell migration, ERK activity, MMP-9 expression, and activation of NF-K kappa B and AP-1 in bladder cancer cells. The effects of IL-5-induced cell responses were confirmed by transfection of IL-5 gene, which demonstrated that p21WAF1 participates in the induction of cell migration, leading to an increase in ERK1/2-mediated MMP-9 expression through activation of NF-kappa B and AP-1 in IL-5-treated bladder cancer cells. These unexpected results provide a theoretical basis for the therapeutic targeting of IL-5 in bladder cancer. (C) 2013 Elsevier Inc All rights reserved. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | Interleukin-5 enhances the migration and invasion of bladder cancer cells via ERK1/2-mediated MMP-9/NF-kappa B/AP-1 pathway: Involvement of the p21WAF1 expression | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.cellsig.2013.06.004 | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, v.25, no.10, pp 2025 - 2038 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000322943700010 | - |
dc.identifier.scopusid | 2-s2.0-84880177554 | - |
dc.citation.endPage | 2038 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 2025 | - |
dc.citation.title | CELLULAR SIGNALLING | - |
dc.citation.volume | 25 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | IL-5 | - |
dc.subject.keywordAuthor | IL-5R alpha | - |
dc.subject.keywordAuthor | p21WAF1 | - |
dc.subject.keywordAuthor | Migration | - |
dc.subject.keywordAuthor | ERK1/2 | - |
dc.subject.keywordAuthor | MMP-9 | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | CYCLIN-DEPENDENT KINASES | - |
dc.subject.keywordPlus | IV COLLAGENASE GENE | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | TUMOR PROGRESSION | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE-9 EXPRESSION | - |
dc.subject.keywordPlus | INFLAMMATORY CYTOKINES | - |
dc.subject.keywordPlus | HUMAN EOSINOPHILS | - |
dc.subject.keywordPlus | URINARY-BLADDER | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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