Human β-defensin 2 may inhibit internalisation of bacillus Calmette-Guerin (BCG) in bladder cancer cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Jung Hoon | - |
dc.contributor.author | Kim, Soon-Ja | - |
dc.contributor.author | Lee, Kyung Mee | - |
dc.contributor.author | Chang, In Ho | - |
dc.date.available | 2019-03-09T01:03:01Z | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 1464-4096 | - |
dc.identifier.issn | 1464-410X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14264 | - |
dc.description.abstract | Objective To investigate whether secretion of human beta-defensin 2 (HBD-2) is induced by bacillus Calmette-Guerin (BCG) and to determine whether HBD-2 affects BCG internalisation in bladder cancer cells. Materials and Methods Reverse transcription-polymerase chain reaction analysis was used to determine whether HBD-2 mRNA increases after incubation with BCG. HBD-2 proteins in 5637 and T24 human bladder cancer cell lines were assayed by enzyme-linked immunosorbent assay. The internalisation rate was evaluated by double immunofluorescence assay and confocal microscopy to test the optimal dose of HBD-2 for BCG internalisation. We also investigated the difference in internalisation rates and cell viability between recombinant HBD-2 protein, anti-HBD-2 antibody, and HBD-2 plus anti-HBD-2 antibody pretreatments. Results BCG induced HBD-2 mRNA expression and HBD-2 production dose and time-dependently in bladder cancer cells and affected BCG internalisation. Pretreatment with recombinant HBD-2 protein lowered internalisation of BCG dose-dependently. Moreover, anti-HBD-2 antibody prevented the effect of HBD-2 on BCG internalisation in bladder cancer cells. The internalisation rate of BCG pretreated with anti-HBD-2 antibody was higher than that in the control in 5637 (P < 0.01) and T24 cells (P < 0.05). The BCG internalisation rate in cells pretreated with anti-HBD-2 antibody plus recombinant HBD-2 protein was higher than that in the control in 5637 (P < 0.01) and T24 cells (P < 0.05). Mycobacterium bovis BCG decreased bladder cancer cell viability, and anti-HBD-2 antibody prevented the inhibitory role of HBD-2 on the anti-proliferative effects of M. bovis BCG in bladder cancer cells Conclusion Bladder cancer cells produce HBD-2 when they are infected by BCG to defend themselves against BCG internalisation, which plays an important role during the initiation and propagation of the immunotherapeutic response in bladder cancer cells. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Human β-defensin 2 may inhibit internalisation of bacillus Calmette-Guerin (BCG) in bladder cancer cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/bju.12196 | - |
dc.identifier.bibliographicCitation | BJU INTERNATIONAL, v.112, no.6, pp 781 - 790 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000324386700019 | - |
dc.identifier.scopusid | 2-s2.0-84884285271 | - |
dc.citation.endPage | 790 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 781 | - |
dc.citation.title | BJU INTERNATIONAL | - |
dc.citation.volume | 112 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | bladder | - |
dc.subject.keywordAuthor | cancer | - |
dc.subject.keywordAuthor | human beta-defensin 2 | - |
dc.subject.keywordAuthor | bacillus Calmette-Guerin | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | MYCOBACTERIUM-BOVIS | - |
dc.subject.keywordPlus | HUMAN BETA-DEFENSIN-2 | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | IMMUNOTHERAPY | - |
dc.subject.keywordPlus | TUBERCULOSIS | - |
dc.subject.keywordPlus | INFECTION | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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