Functional and molecular characterization of novel Hansenula polymorpha genes, HpPMT5 and HpPMT6, encoding protein O-mannosyltransferases
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyunah | - |
dc.contributor.author | Moon, Hye Yun | - |
dc.contributor.author | Lee, Dong-jik | - |
dc.contributor.author | Cheon, Seon Ah | - |
dc.contributor.author | Yoo, Su Jin | - |
dc.contributor.author | Park, Jeong-Nam | - |
dc.contributor.author | Agaphonov, Michael O. | - |
dc.contributor.author | Oh, Doo-Byoung | - |
dc.contributor.author | Kwon, Ohsuk | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.date.available | 2019-03-09T01:38:05Z | - |
dc.date.issued | 2013-09 | - |
dc.identifier.issn | 1087-1845 | - |
dc.identifier.issn | 1096-0937 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14352 | - |
dc.description.abstract | The genome of the thermotolerant methylotrophic yeast Hansenula polymorpha reveals the presence of five PMT homologues (HpPMT1, HpPMT2, HpPMT4, HpPMT5, and HpPMT6) encoding protein O-mannosyltransferases. Here, we report on the systematic characterization of HpPMT5 and HpPMT6, encoding novel PMT1 and PMT2 subfamily members, respectively. Although no apparent growth defects were detected in the Hppmt5 Delta and Hppmt6 Delta single mutants, the single mutants showed dramatic sensitivity to the Pmt1p inhibitor, and the Hppmt1pmt5 Delta and Hppmt1pmt6 Delta double mutants displayed increased susceptibility to cell wall-disturbing reagents. Activation of the cell wall integrity signaling pathway in the double mutant strains was further indicated by the markedly induced phosphorylation of MAP kinases, such as HpMpk1p and HpHog1p. Noticeably, O-mannosylation of the surface glycoproteins HpWsc1p and HpMid2p became severely defective only in the double mutants, supporting the involvement of HpPmt5p and HpPmt6p in O-mannosylation of these sensor proteins. On the other hand, co-immunoprecipitation experiments revealed only marginal interaction between HpPmt5p and HpPmt2p, even in the absence of HpPmt1p. Taken together, our results suggest that the functions of HpPmt5p and HpPmt6p are minor but become crucial upon the loss of HpPmt1p for protein O-mannosylation, which is essential for cell growth, cell wall integrity, and stress resistance in H. polymorpha. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 15 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Functional and molecular characterization of novel Hansenula polymorpha genes, HpPMT5 and HpPMT6, encoding protein O-mannosyltransferases | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.fgb.2013.08.003 | - |
dc.identifier.bibliographicCitation | FUNGAL GENETICS AND BIOLOGY, v.58-59, pp 10 - 24 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000325737000002 | - |
dc.identifier.scopusid | 2-s2.0-84884568752 | - |
dc.citation.endPage | 24 | - |
dc.citation.startPage | 10 | - |
dc.citation.title | FUNGAL GENETICS AND BIOLOGY | - |
dc.citation.volume | 58-59 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | O-mannosylation | - |
dc.subject.keywordAuthor | Pmt proteins | - |
dc.subject.keywordAuthor | Cell wall integrity | - |
dc.subject.keywordAuthor | Hansenula polymorpha | - |
dc.subject.keywordPlus | CELL-WALL INTEGRITY | - |
dc.subject.keywordPlus | PATHOGEN CANDIDA-ALBICANS | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | ASPERGILLUS-FUMIGATUS | - |
dc.subject.keywordPlus | CRYPTOCOCCUS-NEOFORMANS | - |
dc.subject.keywordPlus | ANTIFUNGAL RESISTANCE | - |
dc.subject.keywordPlus | TRICHODERMA-REESEI | - |
dc.subject.keywordPlus | ENZYMATIC-ACTIVITY | - |
dc.subject.keywordPlus | SURFACE SENSORS | - |
dc.subject.keywordPlus | FISSION YEAST | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Mycology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Mycology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.