Cell-Surface Expression of Aspergillus saitoi-Derived Functional alpha-1,2-Mannosidase on Yarrowia lipolytica for Glycan Remodeling
DC Field | Value | Language |
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dc.contributor.author | Moon, Hye Yun | - |
dc.contributor.author | Van, Trinh Luu | - |
dc.contributor.author | Cheon, Seon Ah | - |
dc.contributor.author | Choo, Jinho | - |
dc.contributor.author | Kim, Jeong-Yoon | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.date.available | 2019-03-09T01:40:07Z | - |
dc.date.issued | 2013-08 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.issn | 1976-3794 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14434 | - |
dc.description.abstract | Expression of proteins on the surface of yeast has a wide range of applications, such as development of live vaccines, screening of antibody libraries, and use as whole-cell bio-catalysts. The hemiascomycetes yeast Yarrowia hpolytica has been raised as a potential host for heterologous expression of recombinant proteins. In this study, we report the expression of Aspergillus saitoi alpha-1,2-mannosidase, encoded by the msdS gene, on the cell surface of Y. lipolytica. As the first step to achieve the secretory expression of msdS protein, four different signal sequences-derived from the endogenous Y. lipolytica Lip2 and Xpr2 prepro regions and the heterologous A. niger alpha-amylase and rice a-amylase signal sequences-were analyzed for their secretion efficiency. It was shown that the YlLip2 prepro sequence was most efficient in directing the secretory expression of msdS in fully N-glycosylated forms. The surface display of msdS was subsequently directed by fusing GPI anchoring motifs derived from Y. lipolytica cell wall proteins, YlCwp1p and YlYwp1p, respectively, to the C-terminus of the Lip2 prepro-msdS protein. The expression of actively functional msdS protein on the cell surface was confirmed by western blot, flow cytometry analysis, along with the alpha-1,2-mannosidase activity assay using intact Y. lipolytica cells as the enzyme source. Furthermore, the glycoengineered Y. lipolytica Delta och1 Delta mpo1 strains displaying alpha-1,2-mannosidase were able to convert Man(8)GlcNAc(2) to Man(5)GlcNAc(2) efficiently on their cell-wall mannoproteins, demonstrating its potential used for glycoengineering in vitro or in vivo. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.title | Cell-Surface Expression of Aspergillus saitoi-Derived Functional alpha-1,2-Mannosidase on Yarrowia lipolytica for Glycan Remodeling | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12275-013-3344-x | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.51, no.4, pp 506 - 514 | - |
dc.identifier.kciid | ART001798511 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000323687400014 | - |
dc.identifier.scopusid | 2-s2.0-84883373332 | - |
dc.citation.endPage | 514 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 506 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 51 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Yarrowia lipolytica | - |
dc.subject.keywordAuthor | surface display | - |
dc.subject.keywordAuthor | a-1,2-mannosidase | - |
dc.subject.keywordAuthor | GPI-anchor | - |
dc.subject.keywordPlus | YEAST HANSENULA-POLYMORPHA | - |
dc.subject.keywordPlus | PICHIA-PASTORIS | - |
dc.subject.keywordPlus | PROTEIN PIR | - |
dc.subject.keywordPlus | N-GLYCANS | - |
dc.subject.keywordPlus | DISPLAY | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | GLYCOPROTEINS | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | MUTANT | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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