The anti-adipogenic effects of (-)epigallocatechin gallate are dependent on the WNT/beta-catenin pathway
DC Field | Value | Language |
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dc.contributor.author | Lee, Haeyong | - |
dc.contributor.author | Bae, Sungmin | - |
dc.contributor.author | Yoon, Yoosik | - |
dc.date.available | 2019-03-09T01:41:46Z | - |
dc.date.issued | 2013-07 | - |
dc.identifier.issn | 0955-2863 | - |
dc.identifier.issn | 1873-4847 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14499 | - |
dc.description.abstract | (-)Epigallocatechin gallate (EGCG) is the most abundant catechin in green tea and reportedly has anti-obesity and anti-adipogenic effects. In this study, we determined that the up-regulation of the WNT/beta-catenin pathway is the anti-adipogenic mechanisms of EGCG in 3T3-L1 cells. EGCG treatment down-regulates the expression of major genes involved in the adipogenesis pathway including peroxisome proliferator-activated receptor (PPAR)gamma, CCAAT/enhancer binding protein (C/EBP)alpha, fatty acid binding protein (FABP)4 and fatty acid synthase (FASN), while up-regulating the nuclear level of beta-catenin. Knockdown of beta-catenin using small interfering (si) RNA attenuated the inhibitory effects of EGCG on intracellular lipid accumulation. beta-catenin siRNA transfection also recovered terminal adipocyte markers such as FABP4, FASN, lipoprotein lipase and adiponectin, which were down-regulated by EGCG. The DNA binding activities as well as the expression levels of PPAR gamma and C/EBP alpha, which were down-regulated by EGCG, were significantly restored by beta-catenin siRNA transfection. In addition, we found that EGCG efficiently up-regulates the WNT/beta-catenin pathway. Among the members of the WNT/beta-catenin pathway, the expressions of low density lipoprotein receptor-related protein (LRP)5, LRP6, disheveled (DVL)2 and DVL3 were significantly up-regulated, while AXIN expression was down-regulated by EGCG, and the phosphorylation of glycogen synthase kinase 3 beta was increased. These results suggest that EGCG activates the WNT/beta-catenin pathway, resulting in the up-regulation of beta-catenin, which down-regulates the major genes of the adipogenesis pathway. Taken together, our findings clearly show that the anti-adipogenic effects of EGCG are, at least partially, dependent on the WNT/beta-catenin pathway. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | The anti-adipogenic effects of (-)epigallocatechin gallate are dependent on the WNT/beta-catenin pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jnutbio.2012.09.007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v.24, no.7, pp 1232 - 1240 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000321412600005 | - |
dc.identifier.scopusid | 2-s2.0-84879319769 | - |
dc.citation.endPage | 1240 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1232 | - |
dc.citation.title | JOURNAL OF NUTRITIONAL BIOCHEMISTRY | - |
dc.citation.volume | 24 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Adipogenesis | - |
dc.subject.keywordAuthor | beta-catenin | - |
dc.subject.keywordAuthor | EGCG | - |
dc.subject.keywordAuthor | WNT | - |
dc.subject.keywordAuthor | 3T3-L1 | - |
dc.subject.keywordPlus | ACTIVATED RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | TEA (-)-EPIGALLOCATECHIN GALLATE | - |
dc.subject.keywordPlus | 3T3-L1 PREADIPOCYTES DEPENDS | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | GREEN TEA | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | EPIGALLOCATECHIN-3-GALLATE EGCG | - |
dc.subject.keywordPlus | FUNCTIONAL INTERACTION | - |
dc.subject.keywordPlus | ADIPOSE CONVERSION | - |
dc.subject.keywordPlus | LIPID-ACCUMULATION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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