Characterization of the Gene Encoding Radish (Raphanus sativus L.) PG-inhibiting Protein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, Byung-Ho | - |
dc.contributor.author | Kim, Hun | - |
dc.contributor.author | Lim, Sooyeon | - |
dc.contributor.author | Han, NaRae | - |
dc.contributor.author | Kim, Jongkee | - |
dc.date.available | 2019-03-09T01:57:23Z | - |
dc.date.issued | 2013-06 | - |
dc.identifier.issn | 1226-8763 | - |
dc.identifier.issn | 2465-8588 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14622 | - |
dc.description.abstract | A radish (Raphanus sativus L.) polygalacturonase-inhibiting protein (PGIP) gene was cloned and compared to the PGIP gene (BrPGIP2) from Chinese cabbage (Brassica rapa ssp. pekinensis) in order to gain more information on controlling a disease and improving produce quality. To clone the radish PGIP gene, primers were designed based on conserved sequences of two PGIP genes (BnPGIP1 and BnPGIP2) from rape (B. napus L. ssp. oleifera), Chinese cabbage and Arabidopsis thaliana. PCR cloning was performed with cDNA from the stigma of radish 'Daejinyeoreum' as a template to confirm DNA fragments which were about 600 base pair in size. Sequence analysis revealed 84.1% homology with BrPGIP2 and 70.1% with BnPGIP1. DNA walking was conducted to confirm the open reading frame of 972 bp, and the gene was named RsPGIP1. RsPGIP1 consisting with 323 amino acids (aa) has a high leucine content (54/323) and contains 10 leucine-rich repeat domains, as do most BrPGIPs of Chinese cabbage. The gene expression of RsPGIP1 was induced by abiotic stresses and methyl jasmonate. It showed enrichment in the stigma and the primary root than a leaf. Cloning RsPGIP1 will aid to further apply practices on postharvest quality maintenance and disease control of the root. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC HORTICULTURAL SCIENCE | - |
dc.title | Characterization of the Gene Encoding Radish (Raphanus sativus L.) PG-inhibiting Protein | - |
dc.type | Article | - |
dc.identifier.doi | 10.7235/hort.2013.12211 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY, v.31, no.3, pp 299 - 307 | - |
dc.identifier.kciid | ART001781861 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000322049000006 | - |
dc.identifier.scopusid | 2-s2.0-84940312809 | - |
dc.citation.endPage | 307 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 299 | - |
dc.citation.title | KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY | - |
dc.citation.volume | 31 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | PGIP | - |
dc.subject.keywordAuthor | polygalacturonase | - |
dc.subject.keywordAuthor | postharvest disease | - |
dc.subject.keywordAuthor | produce quality | - |
dc.subject.keywordAuthor | radish | - |
dc.subject.keywordPlus | POLYGALACTURONASE INHIBITOR | - |
dc.subject.keywordPlus | FUNGAL-INFECTION | - |
dc.subject.keywordPlus | PLANT DEFENSE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | ENDOPOLYGALACTURONASE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | RESIDUES | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalWebOfScienceCategory | Horticulture | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.