The alpha-Barrel Tip Region of Escherichia coli TolC Homologs of Vibrio vulnificus Interacts with the MacA Protein to Form the Functional Macrolide-Specific Efflux Pump MacAB-TolC
DC Field | Value | Language |
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dc.contributor.author | Lee, Minho | - |
dc.contributor.author | Kim, Hyun-Lee | - |
dc.contributor.author | Song, Saemee | - |
dc.contributor.author | Joo, Minju | - |
dc.contributor.author | Lee, Seunghwa | - |
dc.contributor.author | Kim, Daeyoung | - |
dc.contributor.author | Hahn, Yoonsoo | - |
dc.contributor.author | Ha, Nam-Chul | - |
dc.contributor.author | Lee, Kangseok | - |
dc.date.available | 2019-03-09T02:00:42Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.issn | 1976-3794 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14747 | - |
dc.description.abstract | TolC and its homologous family of proteins are outer membrane factors that are essential for exporting small molecules and toxins across the outer membrane in Gram-negative bacteria. Two open reading frames in the Vibrio vulnificus genome that encode proteins homologous to Escherichia coli TolC, designated TolCV1 and TolCV2, have 51.3% and 29.6% amino acid identity to TolC, respectively. In this study, we show that TolCV1 and TolCV2 functionally and physically interacted with the membrane fusion protein, MacA, a component of the macrolide-specific MacAB-TolC pump of E. coli. We further show that the conserved residues located at the aperture tip region of the alpha-hairpin of TolCV1 and TolCV2 played an essential role in the formation of the functional MacAB-TolC pump using site-directed mutational analyses. Our findings suggest that these outer membrane factors have conserved tip-to-tip interaction with the MacA membrane fusion protein for action of the drug efflux pump in Gram-negative bacteria. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.title | The alpha-Barrel Tip Region of Escherichia coli TolC Homologs of Vibrio vulnificus Interacts with the MacA Protein to Form the Functional Macrolide-Specific Efflux Pump MacAB-TolC | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12275-013-2699-3 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.51, no.2, pp 154 - 159 | - |
dc.identifier.kciid | ART001761712 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000318198800002 | - |
dc.identifier.scopusid | 2-s2.0-84876833397 | - |
dc.citation.endPage | 159 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 154 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 51 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | MacA | - |
dc.subject.keywordAuthor | MacB | - |
dc.subject.keywordAuthor | TolC | - |
dc.subject.keywordAuthor | TolCV1 | - |
dc.subject.keywordAuthor | TolCV2 | - |
dc.subject.keywordAuthor | Type I secretion system | - |
dc.subject.keywordPlus | MEMBRANE-FUSION PROTEIN | - |
dc.subject.keywordPlus | GRAM-NEGATIVE BACTERIA | - |
dc.subject.keywordPlus | MULTIDRUG EFFLUX | - |
dc.subject.keywordPlus | PSEUDOMONAS-AERUGINOSA | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | PERIPLASMIC COMPONENT | - |
dc.subject.keywordPlus | ANTIBIOTIC EFFLUX | - |
dc.subject.keywordPlus | ATPASE ACTIVITY | - |
dc.subject.keywordPlus | TRANSPORTER | - |
dc.subject.keywordPlus | ACRA | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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