Codon optimization of the human papillomavirus type 58 L1 gene enhances the expression of soluble L1 protein in Saccharomyces cerevisiae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyoung Jin | - |
dc.contributor.author | Kwag, Hye-Lim | - |
dc.contributor.author | Kim, Hong-Jin | - |
dc.date.available | 2019-03-09T02:01:43Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.issn | 1573-6776 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14789 | - |
dc.description.abstract | The effect of codon optimization of L1 gene on the production of the L1 protein of human papillomavirus (HPV) was investigated in a yeast expression system. Saccharomyces cerevisiae was transformed with a plasmid containing either the wild type (WS)-HPV type 58 L1 (HPV58 L1) gene or a codon-optimized (MO)-HPV58 L1 gene. The proportion of soluble L1 protein expressed from MO-HPV58 L1 was significantly higher than that expressed from WS-HPV58 L1. Moreover, the amount of purified MO-HPV58 L1 protein recovered was 2.5-fold higher than the amount of WS-HPV58 L1 protein. Codon optimization of HPV58 L1 gene thus increases the proportion of soluble L1 protein and the amount of purified product that can be used as antigen to generate vaccines. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Codon optimization of the human papillomavirus type 58 L1 gene enhances the expression of soluble L1 protein in Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s10529-012-1097-y | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, v.35, no.3, pp 413 - 421 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000314788400016 | - |
dc.identifier.scopusid | 2-s2.0-84873990868 | - |
dc.citation.endPage | 421 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 413 | - |
dc.citation.title | BIOTECHNOLOGY LETTERS | - |
dc.citation.volume | 35 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Codon optimization | - |
dc.subject.keywordAuthor | Human papillomavirus | - |
dc.subject.keywordAuthor | L1 protein | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordPlus | VIRUS-LIKE PARTICLES | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | USAGE | - |
dc.subject.keywordPlus | DETERMINANTS | - |
dc.subject.keywordPlus | PSEUDOVIRUS | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.