Interleukin-20 Promotes Migration of Bladder Cancer Cells through Extracellular Signal-regulated Kinase (ERK)-mediated MMP-9 Protein Expression Leading to Nuclear Factor (NF-kappa B) Activation by Inducing the Up-regulation of p21(WAF1) Protein Expression
DC Field | Value | Language |
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dc.contributor.author | Lee, Se-Jung | - |
dc.contributor.author | Cho, Seok-Cheol | - |
dc.contributor.author | Lee, Eo-Jin | - |
dc.contributor.author | Kim, Sangtae | - |
dc.contributor.author | Lee, Soo-Bok | - |
dc.contributor.author | Lim, Jung-Hyurk | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.date.available | 2019-03-09T02:03:00Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14843 | - |
dc.description.abstract | The role of inflammatory cytokine interleukin-20 (IL-20) has not yet been studied in cancer biology. Here, we demonstrated up-regulation of both IL-20 and IL-20R1 in muscle-invasive bladder cancer patients. The expressions of IL-20 and IL-20R1 were observed in bladder cancer 5637 and T-24 cells. We found that IL-20 significantly increased the expression of matrix metalloproteinase (MMP)-9 via binding activity of NF-kappa B and AP-1 in bladder cancer cells and stimulated the activation of ERK1/2, JNK, p38 MAPK, and JAK-STAT signaling. Among the pathways examined, only ERK1/2 inhibitor U0126 significantly inhibited IL-20-induced migration and invasion. Moreover, siRNA knockdown of IL-20R1 suppressed migration, invasion, ERK1/2 activation, and NF-kappa B-mediated MMP-9 expression induced by IL-20. Unexpectedly, the cell cycle inhibitor p21(WAF1) was induced by IL-20 treatment without altering cell cycle progression. Blockade of p21(WAF1) function by siRNA reversed migration, invasion, activation of ERK signaling, MMP-9 expression, and activation of NF-kappa B in IL-20-treated cells. In addition, IL-20 induced the activation of I kappa B kinase, the degradation and phosphorylation of I kappa B alpha, and NF-kappa B p65 nuclear translocation, which was regulated by ERK1/2. IL-20 stimulated the recruitment of p65 to the MMP-9 promoter region. Finally, the IL-20-induced migration and invasion of cells was confirmed by IL-20 gene transfection and by addition of anti-IL-20 antibody. This is the first report that p21(WAF1) is involved in ERK1/2-mediated MMP-9 expression via increased binding activity of NF-kappa B, which resulted in the induction of migration in IL-20/IL-20R1 dyad-induced bladder cancer cells. These unexpected results might provide a critical new target for the treatment of bladder cancer. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Interleukin-20 Promotes Migration of Bladder Cancer Cells through Extracellular Signal-regulated Kinase (ERK)-mediated MMP-9 Protein Expression Leading to Nuclear Factor (NF-kappa B) Activation by Inducing the Up-regulation of p21(WAF1) Protein Expression | - |
dc.type | Article | - |
dc.identifier.doi | 10.1074/jbc.M112.410233 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.288, no.8, pp 5539 - 5552 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000315342500028 | - |
dc.identifier.scopusid | 2-s2.0-84874343878 | - |
dc.citation.endPage | 5552 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 5539 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 288 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | IV COLLAGENASE GENE | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | TUMOR PROGRESSION | - |
dc.subject.keywordPlus | CYCLE REGULATORS | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | INFLAMMATORY CYTOKINES | - |
dc.subject.keywordPlus | URINARY-BLADDER | - |
dc.subject.keywordPlus | CDK INHIBITORS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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