Preparation of photoreactive azidophenyl hyaluronic acid derivative: Protein immobilization for medical applications
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyung-Jae | - |
dc.contributor.author | Park, Shin-Hye | - |
dc.contributor.author | Seo, Si-Yoong | - |
dc.contributor.author | Cho, Young-Min | - |
dc.contributor.author | Woo, Hee Dong | - |
dc.contributor.author | Ito, Yoshihiro | - |
dc.contributor.author | Son, Tae-Il | - |
dc.date.available | 2019-03-09T02:36:44Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 1598-5032 | - |
dc.identifier.issn | 2092-7673 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14897 | - |
dc.description.abstract | Photoreactive azidophenyl hyaluronic acid (Az-HA) derivative was synthesized to immobilize proteins such as epidermal growth factor (EGF) and bone morphogenetic protein (BMP). The photosensitizing group, 4-azidoaniline, was introduced to the carboxyl group of hyaluronic acid by a water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). To characterize the synthesized Az-HA, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (H-1 NMR) and ultraviolet/visible (UV/vis) absorbance spectroscopy were performed. We demonstrated protein immobilization by using fluorescein isothiocyanate conjugated albumin (FITCBSA) on Az-HA derivative surfaces that were patterned with micro scale. Cytotoxicity and relative cell proliferation tests of the Az-HA derivative were performed on an MG-63 human osteoblast cell line for biocompatibility. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | POLYMER SOC KOREA | - |
dc.title | Preparation of photoreactive azidophenyl hyaluronic acid derivative: Protein immobilization for medical applications | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s13233-013-1016-7 | - |
dc.identifier.bibliographicCitation | MACROMOLECULAR RESEARCH, v.21, no.2, pp 216 - 220 | - |
dc.identifier.kciid | ART001742287 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000315449700015 | - |
dc.identifier.scopusid | 2-s2.0-84878505182 | - |
dc.citation.endPage | 220 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 216 | - |
dc.citation.title | MACROMOLECULAR RESEARCH | - |
dc.citation.volume | 21 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | hyaluronic acid | - |
dc.subject.keywordAuthor | 4-azidoaniline | - |
dc.subject.keywordAuthor | 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride | - |
dc.subject.keywordAuthor | photoreactive | - |
dc.subject.keywordAuthor | protein immobilization | - |
dc.subject.keywordPlus | TISSUE ENGINEERING SCAFFOLDS | - |
dc.subject.keywordPlus | PHOTO-CROSS-LINKING | - |
dc.subject.keywordPlus | DNA COMPLEX | - |
dc.subject.keywordPlus | AMINO-ACID | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | INSULIN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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