Cartilage Tissue Formation From Dedifferentiated Chondrocytes by Code livery of BMP-2 and SOX-9 Genes Encoding Bicistronic Vector
DC Field | Value | Language |
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dc.contributor.author | Cha, Byung-Hyun | - |
dc.contributor.author | Kim, Jae-Hwan | - |
dc.contributor.author | Kang, Sun-Woong | - |
dc.contributor.author | Do, Hyun-Jin | - |
dc.contributor.author | Jang, Ju-Woong | - |
dc.contributor.author | Choi, Yon Rak | - |
dc.contributor.author | Park, Hansoo | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.contributor.author | Lee, Soo-Hong | - |
dc.date.available | 2019-03-09T02:41:40Z | - |
dc.date.issued | 2013-09 | - |
dc.identifier.issn | 0963-6897 | - |
dc.identifier.issn | 1555-3892 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/15097 | - |
dc.description.abstract | Articular cartilage, when damaged by degenerative disease or trauma, has limited ability for self-repair. Recently, many trials have demonstrated that gene therapy combined with tissue engineering techniques would be a promising approach for cartilage regeneration. Bone morphogenetic protein 2 (BMP-2) is an important signal for upregulation of osteogenesis and chondrogenesis of stem cells. Sex-determining region Y box gene 9 (SOX-9) has also been reported as one of the key transcription factors for chondrogenesis. We hypothesized that codelivery of BMP-2 and SOX-9 genes would result in improved efficiency of recovery of normal chondrogenic properties in dedifferentiated chondrocytes. To this aim, we constructed a bicistronic vector encoding the BMP-2 and SOX-9 genes linked to the "self-cleaving" 2A peptide sequence. After gene delivery to dedifferentiated chondrocytes using a microporator transfection system, we confirmed over 65% delivery efficiency of the BMP-2 and SOX-9 genes. According to RT-PCR analysis and Alcian blue staining, simultaneous delivery of BMP-2/SOX-9 resulted in significantly increased expression of chondrogenesis-related markers (type II collagen and aggrecan) and GAG matrix formation compared with individual delivery of the BMP-2 or SOX-9 gene. Six weeks after in vivo transplantation, BMP-2/SOX-9 genes also showed a significant increase in cartilage formation compared with the BMP-2 or SOX-9 gene. These results demonstrate that codelivery of two chondrogenic lineage-determining genes can enhance normal chondrogenic properties of dedifferentiated chondrocytes followed by improved cartilage formation. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.title | Cartilage Tissue Formation From Dedifferentiated Chondrocytes by Code livery of BMP-2 and SOX-9 Genes Encoding Bicistronic Vector | - |
dc.type | Article | - |
dc.identifier.doi | 10.3727/096368912X647261 | - |
dc.identifier.bibliographicCitation | CELL TRANSPLANTATION, v.22, no.9, pp 1519 - 1528 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000325309000002 | - |
dc.identifier.scopusid | 2-s2.0-84883532271 | - |
dc.citation.endPage | 1528 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1519 | - |
dc.citation.title | CELL TRANSPLANTATION | - |
dc.citation.volume | 22 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Bone morphogenetic protein 2 (BMP-2) | - |
dc.subject.keywordAuthor | Sex-determining region Y box gene 9 (SOX-9) | - |
dc.subject.keywordAuthor | Codelivery | - |
dc.subject.keywordAuthor | Dedifferentiation | - |
dc.subject.keywordAuthor | Cartilage tissue formation | - |
dc.subject.keywordPlus | BONE MORPHOGENETIC PROTEIN-2 | - |
dc.subject.keywordPlus | HUMAN ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | ARTICULAR CHONDROCYTES | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | REDIFFERENTIATION | - |
dc.subject.keywordPlus | CHONDROGENESIS | - |
dc.subject.keywordPlus | COLLAGEN | - |
dc.subject.keywordPlus | REPAIR | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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