Persistent DNA Damage Measured by Comet Assay of Sprague Dawley Rat Lung Cells after Five Days of Inhalation Exposure and 1 Month Post-Exposure to Dispersed Multi-Wall Carbon Nanotubes (MWCNTs) Generated by New MWCNT Aerosol Generation System
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin Sik | - |
dc.contributor.author | Sung, Jae Huyck | - |
dc.contributor.author | Song, Kyung Seuk | - |
dc.contributor.author | Lee, Ji Hyun | - |
dc.contributor.author | Kim, Sun Man | - |
dc.contributor.author | Lee, Gun Ho | - |
dc.contributor.author | Ahn, Kang Ho | - |
dc.contributor.author | Lee, Jong Seong | - |
dc.contributor.author | Shin, Jae Hoon | - |
dc.contributor.author | Park, Jung Duck | - |
dc.contributor.author | Yu, Il Je | - |
dc.date.available | 2019-03-09T02:56:14Z | - |
dc.date.issued | 2012-08 | - |
dc.identifier.issn | 1096-6080 | - |
dc.identifier.issn | 1096-0929 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/15210 | - |
dc.description.abstract | Carbon nanotubes (CNTs) have specific physico-chemical properties that are useful for the electronics, automotive, and construction industries. Yet, despite their many advantages, there is a current lack of available information on the human health and environmental hazards of CNTs. For this reason, the current study investigated the inhalation toxicity potential of multiwall CNTs (MWCNTs). Eight-week-old rats were divided into four groups (10 rats in each group), the fresh-air control (0 mg/m(3)), low-concentration group (0.16 mg/m(3)), middle-concentration group (0.34 mg/m(3)), and high-concentration group (0.94 mg/m(3)), and the whole body was exposed to MWCNTs for 5 days (6h/day). Lung cells were then isolated from five rats in each group on day 0 and 1 month after the 5-day exposure, respectively. The MWCNTs were generated by a newly designed generation system, and the MWCNT concentrations in the exposure chambers monitored in accordance with National Institute for Occupational Safety and Health (NIOSH) 0500 using a membrane filter. The MWCNTs were also sampled for an elemental carbon concentration analysis using a glass filter. The animals exhibited no significant body weight changes, abnormal clinical signs, or mortality during the experiment. A single-cell gel electrophoresis assay (Comet assay) was conducted to determine the DNA damage in lung cells obtained from the right lung. As a result, the Olive tail moments were 23.00 +/- 1.76, 30.39 +/- 1.96, 22.96 +/- 1.26, and 33.98 +/- 2.21 for the control, low-, middle-, and high-concentration groups, respectively, on day 0 postexposure. Meanwhile, 1 month postexposure, the Olive tail moments were 25.00 +/- 2.71, 28.39 +/- 3.55, 22.56 +/- 1.36, and 31.97 +/- 3.16 for the control, low-, middle-, and high-concentration groups, respectively. Thus, the MWCNTs caused a statistically significant increase in lung DNA damage at high concentration (0.94 mg/m(3)) when compared with the negative control group on day 0 and 1 month postexposure. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Persistent DNA Damage Measured by Comet Assay of Sprague Dawley Rat Lung Cells after Five Days of Inhalation Exposure and 1 Month Post-Exposure to Dispersed Multi-Wall Carbon Nanotubes (MWCNTs) Generated by New MWCNT Aerosol Generation System | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/toxsci/kfs161 | - |
dc.identifier.bibliographicCitation | TOXICOLOGICAL SCIENCES, v.128, no.2, pp 439 - 448 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000307698500013 | - |
dc.identifier.scopusid | 2-s2.0-84865354439 | - |
dc.citation.endPage | 448 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 439 | - |
dc.citation.title | TOXICOLOGICAL SCIENCES | - |
dc.citation.volume | 128 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | CNTs | - |
dc.subject.keywordAuthor | MWCNTs | - |
dc.subject.keywordAuthor | inhalation | - |
dc.subject.keywordAuthor | single-cell gel electrophoresis assay (Comet assay) | - |
dc.subject.keywordAuthor | lung DNA damage | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | INTRATRACHEAL INSTILLATION | - |
dc.subject.keywordPlus | PULMONARY TOXICITY | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | SINGLE | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | GENOTOXICITY | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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