Sperm Penetration Assay as an Indicator of Bull Fertility
DC Field | Value | Language |
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dc.contributor.author | Park, Yoo-Jin | - |
dc.contributor.author | Mohamed, El-Sayed A. | - |
dc.contributor.author | Oh, Shin-Ae | - |
dc.contributor.author | Yoon, Sung-Jae | - |
dc.contributor.author | Kwon, Woo-Sung | - |
dc.contributor.author | Kim, Heung-Ruil | - |
dc.contributor.author | Lee, Myeung-Sik | - |
dc.contributor.author | Lee, Kichoon | - |
dc.contributor.author | Pang, Myung-Geol | - |
dc.date.available | 2019-03-09T02:56:30Z | - |
dc.date.issued | 2012-08 | - |
dc.identifier.issn | 0916-8818 | - |
dc.identifier.issn | 1348-4400 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/15218 | - |
dc.description.abstract | To predict the fertility of frozen-thawed bull spermatozoa, a sperm penetration assay (SPA) using zona-free hamster oocytes was optimized, and the assay results were compared with data from field fertility expressed as the non-return rate (NRR). To increase sperm penetration, the spermatozoa were pre-incubated and coincubated with oocytes in media containing various concentrations of heparin (0 to 50 mu g/ml). Coincubation with 10 mu g/ml heparin showed the highest sperm penetration (P<0.05); it is considered to be the optimized SPA method. Sperm fertility index values obtained from WSPA were significantly correlated with the historic average NRR of 46 bulls (P<0.01). To determine the normal range for SPA, we established the lower limits of the sperm fertility index and set the cut-off value at 2.55, at which point the NRR was more than 70 A,, using the receiver operating characteristic curve. The overall accuracy for the 46 bulls was 95.7% (44/46) for both the low and high NRR, with a sensitivity of 95.5% (21/22) and a specificity of 95.8%. This protocol would make it easier to discriminate bulls according to their sperm fertilizing ability. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SOCIETY REPRODUCTION & DEVELOPMENT-SRD | - |
dc.title | Sperm Penetration Assay as an Indicator of Bull Fertility | - |
dc.type | Article | - |
dc.identifier.doi | 10.1262/jrd.11-067H | - |
dc.identifier.bibliographicCitation | JOURNAL OF REPRODUCTION AND DEVELOPMENT, v.58, no.4, pp 461 - 466 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000308055000012 | - |
dc.identifier.scopusid | 2-s2.0-84867873791 | - |
dc.citation.endPage | 466 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 461 | - |
dc.citation.title | JOURNAL OF REPRODUCTION AND DEVELOPMENT | - |
dc.citation.volume | 58 | - |
dc.type.docType | Article | - |
dc.publisher.location | 일본 | - |
dc.subject.keywordAuthor | Bull | - |
dc.subject.keywordAuthor | Male fertility | - |
dc.subject.keywordAuthor | Sperm | - |
dc.subject.keywordAuthor | Sperm penetration assay | - |
dc.subject.keywordAuthor | Prediction | - |
dc.subject.keywordPlus | FREE HAMSTER OVA | - |
dc.subject.keywordPlus | PROTEIN-TYROSINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | ZONA-PELLUCIDA BINDING | - |
dc.subject.keywordPlus | IN-VITRO PENETRATION | - |
dc.subject.keywordPlus | FROZEN-THAWED SEMEN | - |
dc.subject.keywordPlus | BOVINE SPERM | - |
dc.subject.keywordPlus | NONRETURN RATE | - |
dc.subject.keywordPlus | SPERMATOZOA | - |
dc.subject.keywordPlus | OOCYTES | - |
dc.subject.keywordPlus | FERTILIZATION | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Dairy & Animal Science | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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