Synthesis of Bioactive Microcapsules Using a Microfluidic Device
DC Field | Value | Language |
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dc.contributor.author | Kim, Byeong Il | - |
dc.contributor.author | Jeong, Soon Woo | - |
dc.contributor.author | Lee, Kyoung G. | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Park, Jung Youn | - |
dc.contributor.author | Song, Jae Jun | - |
dc.contributor.author | Lee, Seok Jae | - |
dc.contributor.author | Lee, Chang-Soo | - |
dc.date.available | 2019-03-09T02:56:39Z | - |
dc.date.issued | 2012-08 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/15224 | - |
dc.description.abstract | Bioactive microcapsules containing Bacillus thuringiensis (BT) spores were generated by a combination of a hydro gel, microfluidic device and chemical polymerization method. As a proof-of-principle, we used BT spores displaying enhanced green fluorescent protein (EGFP) on the spore surface to spatially direct the EGFP-presenting spores within microcapsules. BT spore-encapsulated microdroplets of uniform size and shape are prepared through a flow-focusing method in a microfluidic device and converted into microcapsules through hydrogel polymerization. The size of microdroplets can be controlled by changing both the dispersion and continuous flow rate. Poly(N-isoproplyacrylamide) (PNIPAM), known as a hydrogel material, was employed as a biocompatible material for the encapsulation of BT spores and long-term storage and outstanding stability. Due to these unique properties of PNIPAM, the nutrients from Luria-Bertani complex medium diffused into the microcapsules and the microencapsulated spores germinated into vegetative cells under adequate environmental conditions. These results suggest that there is no limitation of transferring low-molecular-weight-substrates through the PNIPAM structures, and the viability of microencapsulated spores was confirmed by the culture of vegetative cells after the germinations. This microfluidic-based microencapsulation methodology provides a unique way of synthesizing bioactive microcapsules in a one-step process. This microfluidic-based strategy would be potentially suitable to produce microcapsules of various microbial spores for on-site biosensor analysis. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Synthesis of Bioactive Microcapsules Using a Microfluidic Device | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/s120810136 | - |
dc.identifier.bibliographicCitation | SENSORS, v.12, no.8, pp 10136 - 10147 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000308212100012 | - |
dc.identifier.scopusid | 2-s2.0-84865400491 | - |
dc.citation.endPage | 10147 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 10136 | - |
dc.citation.title | SENSORS | - |
dc.citation.volume | 12 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | microcapsulation | - |
dc.subject.keywordAuthor | NIPAM | - |
dc.subject.keywordAuthor | hydrogel | - |
dc.subject.keywordAuthor | microfluidic device | - |
dc.subject.keywordAuthor | spore | - |
dc.subject.keywordPlus | E. COLI | - |
dc.subject.keywordPlus | MICRODROPLETS | - |
dc.subject.keywordPlus | ENCAPSULATION | - |
dc.subject.keywordPlus | EXOSPORIUM | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Engineering, Electrical & Electronic | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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