Aptamer-conjugated live human immune cell based biosensors for the accurate detection of C-reactive protein
DC Field | Value | Language |
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dc.contributor.author | Hwang, Jangsun | - |
dc.contributor.author | Seo, Youngmin | - |
dc.contributor.author | Jo, Yeonho | - |
dc.contributor.author | Son, Jaewoo | - |
dc.contributor.author | Choi, Jonghoon | - |
dc.date.available | 2019-01-22T14:22:46Z | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1687 | - |
dc.description.abstract | C-reactive protein (CRP) is a pentameric protein that is present in the bloodstream during inflammatory events, e.g., liver failure, leukemia, and/or bacterial infection. The level of CRP indicates the progress and prognosis of certain diseases; it is therefore necessary to measure CRP levels in the blood accurately. The normal concentration of CRP is reported to be 1-3 mg/L. Inflammatory events increase the level of CRP by up to 500 times; accordingly, CRP is a biomarker of acute inflammatory disease. In this study, we demonstrated the preparation of DNA aptamer-conjugated peripheral blood mononuclear cells (Apt-PBMCs) that specifically capture human CRP. Live PBMCs functionalized with aptamers could detect different levels of human CRP by producing immune complexes with reporter antibody. The binding behavior of Apt-PBMCs toward highly concentrated CRP sites was also investigated. The immune responses of Apt-PBMCs were evaluated by measuring TNF-alpha secretion after stimulating the PBMCs with lipopolysaccharides. In summary, engineered Apt-PBMCs have potential applications as live cell based biosensors and for in vitro tracing of CRP secretion sites. | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Aptamer-conjugated live human immune cell based biosensors for the accurate detection of C-reactive protein | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/srep34778 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000385179500001 | - |
dc.identifier.scopusid | 2-s2.0-84990066701 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | INTERLEUKIN-6 | - |
dc.subject.keywordPlus | MARKERS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | CANCER | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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