Effects of carboxypeptidase B treatment and elevated temperature on recombinant monoclonal antibody charge variants in cation-exchange chromatography analysis
- Authors
- Kim, Do Gyun; Kim, Hyoung Jin; Kim, Hong-Jin
- Issue Date
- Oct-2016
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Monoclonal antibody; Charge variant; Cation-exchange chromatography; Carboxypeptidase; C-terminal lysine
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.39, no.10, pp 1472 - 1481
- Pages
- 10
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 39
- Number
- 10
- Start Page
- 1472
- End Page
- 1481
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1747
- DOI
- 10.1007/s12272-016-0818-5
- ISSN
- 0253-6269
1976-3786
- Abstract
- Charge variants (acidic and basic) of recombinant monoclonal antibodies (Mabs) have received much attention due to their potential biological effects. C-terminal lysine variants are common in Mabs and their proportion is affected by the manufacturing process. In the present study, changes of trastuzumab charge variants brought about by carboxypeptidase B treatment and subsequent storage at 8 or 37 A degrees C for up to 24 h were monitored by cation-exchange chromatography analysis to investigate the effects of C-terminal lysine cleavage and its subsequent reaction at 8 or 37 A degrees C. C-terminal lysine cleavage at 8 A degrees C reduced the fraction of basic species and had little effect on the fraction of acidic species. Analysis of individual peaks demonstrated that C-terminal lysine cleavage induced both increases and decreases in individual acidic variants, with the result that there was little overall change in the overall proportion of acidic species. It appeared that most of the basic variant Mab molecules but only a fraction of the acidic variant molecules had C-terminal lysines. Increasing the temperature to 37 A degrees C appeared to increase the fraction of acidic species and decrease main species significantly, without a similar change in basic species. These results indicate that length of exposure to elevated temperature is a critical consideration in charge variant analysis.
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