Lytic transglycosylase contributes to the survival of lipooligosaccharide-deficient, colistin-dependent Acinetobacter baumannii
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, J.-Y. | - |
dc.contributor.author | Lee, H. | - |
dc.contributor.author | Park, M. | - |
dc.contributor.author | Cha, Chang-Jun | - |
dc.contributor.author | Shin, D. | - |
dc.contributor.author | Ko, K.S. | - |
dc.date.available | 2019-05-28T02:49:16Z | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 1198-743X | - |
dc.identifier.issn | 1469-0691 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/18439 | - |
dc.description.abstract | Objectives: The phenomenon of colistin dependence in Acinetobacter baumannii has been described in a situation in which colistin is now considered as the last resort for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. In this study, we aimed to reveal a gene associated with colistin dependence in A. baumannii. Methods: The colistin-dependent A. baumannii H08-391D strain was isolated from a patient, and target gene-inactivation mutants were constructed. We investigated the effects of target gene on colistin dependence with quantitative real-time PCR and endotoxin assay. Also, we observed the change of cell morphology by electron microscopy. Results: The expression of ACICU_02898, encoding a soluble lytic transglycosylase associated with cell-wall degradation and recycling, was increased by eight-to 42-fold in colistin-dependent mutants, and deletion of ACICU_02898 in a colistin-dependent strain led to colistin susceptibility (MIC = 8 mg/L). Endotoxin activity was significantly low in a colistin-dependent derivative ACICU_02898-inactivated mutant and a complemented mutant. In addition, the ACICU_02898-inactivated mutant showed a highly reduced growth rate. The colistin-dependent derivative and ACICU_02898-inactivated mutant showed clearly distinguished absorption profiles in the red/green fluorescence dot blot with regard to their membrane potential. Electron microscopy revealed that the deletion mutant cells were elongated compared to the colistin-susceptible wild-type strain and colistin-dependent strain. Conclusions: A colistin-dependent A. baumannii strain exhibited a deficiency in its outer membrane integrity and high expression of lytic transglycosylase was required for survival. This study reveals why the colistin-dependent mutant can tolerate high antibiotic concentrations. © 2019 European Society of Clinical Microbiology and Infectious Diseases | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Lytic transglycosylase contributes to the survival of lipooligosaccharide-deficient, colistin-dependent Acinetobacter baumannii | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.cmi.2019.02.004 | - |
dc.identifier.bibliographicCitation | Clinical Microbiology and Infection, v.25, no.9, pp 1156.e1 - 1156.e7 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000481643000018 | - |
dc.identifier.scopusid | 2-s2.0-85062637665 | - |
dc.citation.endPage | 1156.e7 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1156.e1 | - |
dc.citation.title | Clinical Microbiology and Infection | - |
dc.citation.volume | 25 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Acinetobacter baumannii | - |
dc.subject.keywordAuthor | Colistin dependence | - |
dc.subject.keywordAuthor | Lytic transglycosylase | - |
dc.subject.keywordAuthor | relA | - |
dc.subject.keywordAuthor | spoT | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | RESISTANT | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | DRUG | - |
dc.relation.journalResearchArea | Infectious Diseases | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Infectious Diseases | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.