Cytoplasmic HMGB1 and HMGB1-Beclin1 complex are increased in radioresistant oral squamous cell carcinoma
DC Field | Value | Language |
---|---|---|
dc.contributor.author | HYUNJIN, MIN | - |
dc.contributor.author | Suh, K.D. | - |
dc.contributor.author | Lee, Y.H. | - |
dc.contributor.author | Kim, K.S. | - |
dc.contributor.author | Mun, S.-K. | - |
dc.contributor.author | Lee, S.Y. | - |
dc.date.available | 2019-05-28T03:32:54Z | - |
dc.date.issued | 2019-04 | - |
dc.identifier.issn | 0266-4356 | - |
dc.identifier.issn | 1532-1940 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/18485 | - |
dc.description.abstract | Cytoplasmic high mobility group box 1 (HMGB1) is an autophagy regulator, and autophagy is important in the radioresistance of various solid cancers. We evaluated the degree of autophagy and cytoplasmic HMGB1 in radioresistant oral squamous cell carcinoma (SCC) by culturing the SCC15 and quasiliquid layer 1 (QLL1) SCC cell lines that originate from cancer of the oral tongue and a metastatic lymph node, respectively, and then delivered radiation to induce radioresistance to cells. We then compared the degree of autophagy between non-irradiated control and radioresistant cancer cells using a western blot assay. We also compared the total and cytoplasmic concentrations of HMGB1 between the non-irradiated control and radioresistant cancer cells by western blot assay, and extracellular concentrations of HMGB1 with an enzyme-linked immunosorbent assay (ELISA). Formation of an HMGB1-Beclin1 complex was evaluated by immunofluorescence and co-immunoprecipitation assays. Autophagy increased in the radioresistant SCC15 cells (compared with non-irradiated control SCC15 cells) but not in the radioresistant QLL1 cells. The total amount of HMGB1 expression within cells did not differ; however, the degree of cytoplasmic HMGB1 expression was higher in radioresistant SCC15 cells than in non-irradiated control SCC15 cells. The HMGB1-Beclin1 complex, which is a main regulator of autophagy, was also increased in radioresistant SCC15 cells compared with non-irradiated control SCC15 cells. Autophagy flux and cytoplasmic HMGB1-Beclin1 increased after the acquisition of radioresistance in oral SCC. © 2019 The British Association of Oral and Maxillofacial Surgeons | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Churchill Livingstone | - |
dc.title | Cytoplasmic HMGB1 and HMGB1-Beclin1 complex are increased in radioresistant oral squamous cell carcinoma | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bjoms.2019.01.011 | - |
dc.identifier.bibliographicCitation | British Journal of Oral and Maxillofacial Surgery, v.57, no.3, pp 219 - 225 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000465553600005 | - |
dc.identifier.scopusid | 2-s2.0-85061039696 | - |
dc.citation.endPage | 225 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 219 | - |
dc.citation.title | British Journal of Oral and Maxillofacial Surgery | - |
dc.citation.volume | 57 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스코트랜드 | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | HMGB1 | - |
dc.subject.keywordAuthor | oral squamous cell carcinoma | - |
dc.subject.keywordAuthor | radioresistance | - |
dc.relation.journalResearchArea | Dentistry, Oral Surgery & Medicine | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Dentistry, Oral Surgery & Medicine | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.