Fabrication of electrochemical biosensor consisted of multi-functional DNA structure/porous au nanoparticle for avian influenza virus (H5N1) in chicken serum
DC Field | Value | Language |
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dc.contributor.author | Lee, Taek | - |
dc.contributor.author | Park, Sun Yong | - |
dc.contributor.author | Jang, Hongje | - |
dc.contributor.author | Kim, Ga-Hyeon | - |
dc.contributor.author | Lee, Yeonju | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Mohammadniaei, Mohsen | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.contributor.author | Min, Junhong | - |
dc.date.available | 2019-05-28T03:34:58Z | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 0928-4931 | - |
dc.identifier.issn | 1873-0191 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/18555 | - |
dc.description.abstract | Avian influenza virus (AIV) is one of the most harmful pathogens to living things due to its fast infection, various mutations, and dangerous symptoms. In this study, we fabricated a label-free AIV H5N1 biosensor composed of multi-functional DNA structure on a porous Au nanoparticles (pAuNPs) fabricated electrode using the electrochemical (EC) technique. As a multi-functional bioprobe, the DNA 3 way-junction (3WJ) was introduced. Each fragment of DNA 3WJ was rolled to recognition part (hemagglutinin (HA) protein detection aptamer), EC signal generation part (horseradish peroxidase (HRP)-mimicked DNAzyme), and immobilization part (Thiol group). Each fragment was assembled in order to form the DNA 3WJ for AI detection and the assembled structure was confirmed by native-tris boric acid magnesium polyacrylamide gel electrophoresis (TBM-PAGE). Moreover, in order to increase the electrochemical signal sensitivity, pAuNPs were synthesized. The property of pAuNPs was investigated by field emission scanning electron microscopy (FE-SEM), Transmission electron microscopy (TEM), Ultraviolet–visible (UV-VIS) spectroscopy and zeta potential analysis. The DNA 3WJ on pAuNPs-modified Au electrode was then prepared using the layer-by-layer (LbL) assembly method. FE-SEM and atomic force microscopy (AFM) were used to investigate the surface morphology. Cyclic voltammetry (CV) was carried out to confirm the HA protein binding to DNA 3WJ-modified electrode. Moreover, The HA protein can be detected 1 pM in HEPES solution and 1 pM in diluted-chicken serum, respectively. The present study showed label-free, simple fabrication, and easy-to-tailor detection elements for AIV. The present biosensor can be a powerful candidate for various virus detection platforms. © 2019 Elsevier B.V. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Fabrication of electrochemical biosensor consisted of multi-functional DNA structure/porous au nanoparticle for avian influenza virus (H5N1) in chicken serum | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.msec.2019.02.001 | - |
dc.identifier.bibliographicCitation | Materials Science and Engineering C, v.99, pp 511 - 519 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000463121200052 | - |
dc.identifier.scopusid | 2-s2.0-85061095732 | - |
dc.citation.endPage | 519 | - |
dc.citation.startPage | 511 | - |
dc.citation.title | Materials Science and Engineering C | - |
dc.citation.volume | 99 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Avian influenza virus detection | - |
dc.subject.keywordAuthor | Hemagglutinin | - |
dc.subject.keywordAuthor | Multi-functional DNA structure | - |
dc.subject.keywordAuthor | DNA 3 way-junction | - |
dc.subject.keywordAuthor | Electrochemical biosensor | - |
dc.subject.keywordAuthor | Porous Au nanoparticle | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | POROUS GOLD | - |
dc.subject.keywordPlus | APTASENSOR | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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