Deacetylase activity-independent transcriptional activation by HDAC2 during TPA-induced HL-60 cell differentiation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Hyeonsoo | - |
dc.contributor.author | Kim, Ji-Young | - |
dc.contributor.author | Kim, Kee-Beom | - |
dc.contributor.author | Chae, Yun-Cheol | - |
dc.contributor.author | Hahn, Yoonsoo | - |
dc.contributor.author | Kim, Jung-Woong | - |
dc.contributor.author | Seo, Sang-Beom | - |
dc.date.available | 2019-03-07T04:35:06Z | - |
dc.date.issued | 2018-08 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1877 | - |
dc.description.abstract | The human myeloid leukemia cell line HL-60 differentiate into monocytes following treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). However, the mechanism underlying the differentiation of these cells in response to TPA has not been fully elucidated. In this study, we performed ChIP-seq profiling of RNA Pol II, HDAC2, Acetyl H3 (AcH3), and H3K27me3 and analyzed differential chromatin state changes during TPA-induced differentiation of HL-60 cells. We focused on atypically active genes, which showed enhanced H3 acetylation despite increased HDAC2 recruitment. We found that HDAC2 positively regulates the expression of these genes in a histone deacetylase activity-independent manner. HDAC2 interacted with and recruited paired box 5 (PAX5) to the promoters of the target genes and regulated HL-60 cell differentiation by PAX5-mediated gene activation. Taken together, these data elucidated the specific-chromatin status during HL-60 cell differentiation following TPA exposure and suggested that HDAC2 can activate transcription of certain genes through interactions with PAX5 in a deacetylase activity-independent pathway. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Deacetylase activity-independent transcriptional activation by HDAC2 during TPA-induced HL-60 cell differentiation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0202935 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.13, no.8 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000442722500050 | - |
dc.identifier.scopusid | 2-s2.0-85052208861 | - |
dc.citation.number | 8 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 13 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | ACUTE MYELOID-LEUKEMIA | - |
dc.subject.keywordPlus | H3K9 METHYLTRANSFERASE G9A | - |
dc.subject.keywordPlus | TRANS-RETINOIC ACID | - |
dc.subject.keywordPlus | VALPROIC ACID | - |
dc.subject.keywordPlus | B-CELLS | - |
dc.subject.keywordPlus | PAX5 EXPRESSION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | LSD1 | - |
dc.subject.keywordPlus | THERAPY | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.