Electrochemical immunosensor for highly sensitive and quantitative detection of tumor necrosis factor-alpha in human serum
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yagati, Ajay Kumar | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.contributor.author | Min, Junhong | - |
dc.date.available | 2019-03-07T04:35:18Z | - |
dc.date.issued | 2018-08 | - |
dc.identifier.issn | 1567-5394 | - |
dc.identifier.issn | 1878-562X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1898 | - |
dc.description.abstract | Highly sensitive and selective biosensors for accurate determination of specific protein biomarkers at low levels in serum are a prerequisite for the present healthcare systems. Therefore, here we developed a label-free impedimetric method for tumor necrosis factor-alpha (TNE-alpha) detection using reduced graphene oxide (RGO) with gold nanoparticles (AuNP) on an indium tin oxide (ITO) microdisk electrodes. The detection mechanism relies on resistance change occurs due to [Fe(CN)(6)](3-/4-) redox probe movement towards the conductive channels of the AuNP-RGO films gated by the recognition of the target biomarker by its anti-TNE-alpha antibody. The conductivity of the AuNP-RGO structures enhanced by 10-fold in comparison with bare electrode chips. The observed resistance changes at 2 Hz (Delta R-at 2Hz) enabled the quantification of various concentrations of TNF-alpha in human serum (CHS-TNF-alpha). The antibody-adsorbed electrode showed a good increment of resistance change (Delta R) with additions of antigen concentration. The sensor possesses a linear range of 1-1000 pg/ml had a detection limit of 0.67 pg/ml (38.51 fM) and 0.78 pg/ml (44.83 fM) in PBS and human serum, respectively. (C) 2018 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | Electrochemical immunosensor for highly sensitive and quantitative detection of tumor necrosis factor-alpha in human serum | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bioelechem.2018.03.007 | - |
dc.identifier.bibliographicCitation | BIOELECTROCHEMISTRY, v.122, pp 93 - 102 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000436649600011 | - |
dc.identifier.scopusid | 2-s2.0-85044469145 | - |
dc.citation.endPage | 102 | - |
dc.citation.startPage | 93 | - |
dc.citation.title | BIOELECTROCHEMISTRY | - |
dc.citation.volume | 122 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Reduced graphene oxide | - |
dc.subject.keywordAuthor | Nanoparticle | - |
dc.subject.keywordAuthor | Tumor necrosis factor-alpha | - |
dc.subject.keywordAuthor | Impedance spectroscopy | - |
dc.subject.keywordAuthor | Biomarker | - |
dc.subject.keywordAuthor | Human serum | - |
dc.subject.keywordPlus | REDUCED GRAPHENE OXIDE | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | ELECTRODE | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | NANOCOMPOSITE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.