Purification and characterization of polyphenol oxidase from fresh ginseng
DC Field | Value | Language |
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dc.contributor.author | Kim, J.-J. | - |
dc.contributor.author | Kim, W.-Y. | - |
dc.date.available | 2019-05-29T03:37:11Z | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 1226-8453 | - |
dc.identifier.issn | 2093-4947 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/19830 | - |
dc.description.abstract | Polyphenol oxidase (PPO) was purified from fresh ginseng roots using acetone precipitation, carboxymethyl (CM)-Sepharose chromatography, and phenyl-Sepharose chromatography. Two isoenzymes (PPO 1 and PPO 2) were separated using an ion-exchange column with CM-Sepharose. PPO 1 was purified up to 13.2-fold with a 22.6% yield. PPO 2 bound to CM-Sepharose, eluted with NaCl, and was purified up to 22.5-fold with a 17.4% yield. PPO 2 was further chromatographed on phenyl-Sepharose. The molecular weight of the purified PPO 2 from fresh ginseng was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was about 40 kDa. The optimum temperature and pH were 20°C and 7.0, respectively, using catechol as a substrate. Pyrogallol showed the highest substrate specificity. The effect of a PPO inhibitor showed that its activity increased slightly in the presence of a low concentration of citric acid. High concentrations of acidic compounds and sulfite agents significantly inhibited purified ginseng PPO 2. © The Korean Society of Ginseng. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Purification and characterization of polyphenol oxidase from fresh ginseng | - |
dc.type | Article | - |
dc.identifier.doi | 10.5142/jgr.2013.37.117 | - |
dc.identifier.bibliographicCitation | Journal of Ginseng Research, v.37, no.1, pp 117 - 123 | - |
dc.identifier.kciid | ART001736951 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000313931400014 | - |
dc.identifier.scopusid | 2-s2.0-84872260208 | - |
dc.citation.endPage | 123 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 117 | - |
dc.citation.title | Journal of Ginseng Research | - |
dc.citation.volume | 37 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Carboxymethyl-sepharose | - |
dc.subject.keywordAuthor | Catechol | - |
dc.subject.keywordAuthor | Panax ginseng | - |
dc.subject.keywordAuthor | Polyphenol oxidase | - |
dc.subject.keywordAuthor | Purification | - |
dc.subject.keywordPlus | Panax ginseng | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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