Genome-Wide Differential Gene Expression Profiles in Broiler Chickens with Gangrenous Dermatitis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Duk Kyung | - |
dc.contributor.author | Lillehoj, Hyun S. | - |
dc.contributor.author | Lee, Kyung Woo | - |
dc.contributor.author | Jang, Seung Ik | - |
dc.contributor.author | Neumann, Anthony P. | - |
dc.contributor.author | Siragusa, Gregory R. | - |
dc.contributor.author | Lillehoj, Erik P. | - |
dc.contributor.author | Hong, Yeong Ho | - |
dc.date.available | 2019-05-29T05:33:53Z | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 0005-2086 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20004 | - |
dc.description.abstract | Gangrenous dermatitis (GD) is a disease of poultry characterized by necrosis of the skin and severe cellulitis of the subcutaneous tissues caused by infection with Clostridium septicum (CS) and/or Clostridium perfringens (CP) type A. While GD causes significant morbidity, mortality, and economic loss to the poultry industry, the fundamental mechanisms underlying this host-pathogen interaction are relatively unknown. This study used comparative global gene expression microarray analysis of GD-affected and clinically healthy chickens from a recent GD outbreak to glean insights into the molecular and cellular changes associated with this disease process. Histopathologic and immunohistochemical analyses confirmed extensive muscle damage and prominent leukocyte infiltration in the skin of GD-affected birds but not in healthy controls. The levels of mRNAs in the skin and underlying muscle corresponding to 952 microarray elements were altered in GD-afflicted birds compared with healthy controls, with 468 being increased and 484 decreased. From these, a subset of 386 genes was identified and used for biologic function and pathway analyses. The biologic functions that were most significantly associated with the differentially expressed genes were "inflammatory response" and "cellular growth and proliferation" classified under the categories of "disease and disorders" and "molecular and cellular functions," respectively. The biologic pathway that was most significantly associated with the differentially expressed genes was the nuclear factor-erythroid 2-related factor 2 (NRF2)-mediated oxidative stress pathway. Finally, in vitro infection of chicken macrophages with CS or CP modified the levels of mRNAs encoding interferon (IFN)-alpha, IFN-gamma, interleukin (IL)-1 beta, IL-6, IL-12p40, tumor necrosis factor superfamily 15 (downregulated), IL-8, and IL-10 (upregulated), thus confirming the suppressive effect of GD on the chicken immune system. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER ASSOC AVIAN PATHOLOGISTS | - |
dc.title | Genome-Wide Differential Gene Expression Profiles in Broiler Chickens with Gangrenous Dermatitis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1637/10069-013112-Reg.1 | - |
dc.identifier.bibliographicCitation | AVIAN DISEASES, v.56, no.4, pp 670 - 679 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000313135800005 | - |
dc.identifier.scopusid | 2-s2.0-84871814286 | - |
dc.citation.endPage | 679 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 670 | - |
dc.citation.title | AVIAN DISEASES | - |
dc.citation.volume | 56 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | gangrenous dermatitis | - |
dc.subject.keywordAuthor | Clostridium perfringens | - |
dc.subject.keywordAuthor | Clostridium septicum | - |
dc.subject.keywordAuthor | differentially expressed genes | - |
dc.subject.keywordAuthor | NRF2-mediated oxidative stress pathway | - |
dc.subject.keywordAuthor | inflammatory response | - |
dc.subject.keywordPlus | CLOSTRIDIUM SEPTICUM INFECTION | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL REGULATION | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | CYTOKINE RESPONSES | - |
dc.subject.keywordPlus | COMMERCIAL TURKEYS | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | GAS-GANGRENE | - |
dc.subject.keywordPlus | ALPHA-TOXIN | - |
dc.subject.keywordPlus | CELL-GROWTH | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.