Genome Variations of Evolved Escherichia coli ET8 With a Rhodopsin-Based Phototrophic Metabolism
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun Aaron | - |
dc.contributor.author | Kim, Hyun Ju | - |
dc.contributor.author | Lee, Min Ju | - |
dc.contributor.author | Park, Jihoon | - |
dc.contributor.author | Choi, Ah Reum | - |
dc.contributor.author | Jeong, Haeyoung | - |
dc.contributor.author | Jung, Kwang-Hwan | - |
dc.contributor.author | Kim, Pil | - |
dc.contributor.author | Lee, Sang Jun | - |
dc.date.available | 2019-03-07T04:38:40Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.issn | 1860-6768 | - |
dc.identifier.issn | 1860-7314 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/2012 | - |
dc.description.abstract | We reported that the phototrophic metabolism via plasmid-originated Gloeobacter rhodopsin(GR)-expression is improved in Escherichia coli ET5 harboring pKJ606-GR by a genomic point mutation (dgcQ(C1082A)) encoding a transmembrane cell signaling protein (Microb. Cell Fact. 16:111, 2017). Another evolved descendant is isolated from the chemostat, and the genome variation of the strain named ET8 harboring pKJ606-GR is investigated in this study. Whole genome sequencing analysis identifies a single point mutation (C3831976A) located in the non-coding upstream region of kdtA and an IS4 insertional mutation at galU(G706) without any mutations in the plasmid. ET8 strain shows enhanced kdtA transcription and no growth in the D-galactose or lactose sole carbon sourced minimal media. Size of ET8 strain are almost identical to that of the ancestor. Phototrophic growth and proton pumping in ET8 expressing GR (ET8+GR) are increased 1.5-fold and threefold, respectively, compared with those in the ancestor (W3110+GR). To verify the effects of the genomic mutations, either the kdtA-upregulation or the galU-disruption is conducted in the ancestor. Both the kdtA-upregulation and the galU-disruption result in the drastic increases of proton-pumping. The physiological properties arising from the genomic variations of the evolved host with the new phototrophic metabolism are further discussed. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Genome Variations of Evolved Escherichia coli ET8 With a Rhodopsin-Based Phototrophic Metabolism | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/biot.201700497 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY JOURNAL, v.13, no.7 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000437281600002 | - |
dc.identifier.scopusid | 2-s2.0-85044399821 | - |
dc.citation.number | 7 | - |
dc.citation.title | BIOTECHNOLOGY JOURNAL | - |
dc.citation.volume | 13 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | adaptive laboratory evolution | - |
dc.subject.keywordAuthor | chemotroph | - |
dc.subject.keywordAuthor | kdtA-upregulation | - |
dc.subject.keywordAuthor | galU-disruption | - |
dc.subject.keywordAuthor | Gloeobacter rhodopsin | - |
dc.subject.keywordAuthor | phototroph | - |
dc.subject.keywordPlus | COLANIC ACID | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | ATP | - |
dc.subject.keywordPlus | PROTEORHODOPSIN | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | STRESS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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