Evaluation of monoclonal antibody-based immunohistochemistry for the detection of European and North American Porcine reproductive and respiratory syndrome virus and a comparison with in situ hybridization and reverse transcription polymerase chain reaction
DC Field | Value | Language |
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dc.contributor.author | Han, Kiwon | - |
dc.contributor.author | Seo, Hwi Won | - |
dc.contributor.author | Oh, Yeonsu | - |
dc.contributor.author | Kang, Ikjae | - |
dc.contributor.author | Park, Changhoon | - |
dc.contributor.author | Kang, Sang Hoon | - |
dc.contributor.author | Kim, Sung-Hoon | - |
dc.contributor.author | Lee, Bog-Hieu | - |
dc.contributor.author | Kwon, Byungjoon | - |
dc.contributor.author | Chae, Chanhee | - |
dc.date.available | 2019-05-29T05:41:33Z | - |
dc.date.issued | 2012-07 | - |
dc.identifier.issn | 1040-6387 | - |
dc.identifier.issn | 1943-4936 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20225 | - |
dc.description.abstract | The objective of the present study was to compare the ability of 2 monoclonal antibodies (mAbs; SDOW17 and SR30) to detect types 1 and 2 Porcine reproductive and respiratory syndrome virus (PRRSV) in formalin-fixed, paraffin-embedded (FFPE) lung tissues by immunohistochemistry (IHC) and to compare the immunohistochemical results with in situ hybridization (ISH) and reverse transcription nested polymerase chain reaction (RT-nPCR) detection techniques. Lungs from 30 experimentally infected pigs (15 pigs with each genotype of PRRSV) and 20 naturally infected pigs (10 pigs with each genotype of PRRSV) with types 1 and 2 PRRSV, respectively, were used for the IHC, ISH, and RT-nPCR analyses. The SR30 mAb-based IHC detected significantly more type 1 PRRSV-positive cells in the accessory and caudal lobes from the experimentally infected pigs at 7 (P = 0.025) and 14 (P = 0.018) days postinoculation, respectively, compared to the SDOW17 mAb-based IHC. The results demonstrated that SR30 mAb-based IHC is useful for detecting both types 1 and 2 PRRSV antigen in FFPE lung tissues. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.title | Evaluation of monoclonal antibody-based immunohistochemistry for the detection of European and North American Porcine reproductive and respiratory syndrome virus and a comparison with in situ hybridization and reverse transcription polymerase chain reaction | - |
dc.type | Article | - |
dc.identifier.doi | 10.1177/1040638712446507 | - |
dc.identifier.bibliographicCitation | JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, v.24, no.4, pp 719 - 724 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000307118700011 | - |
dc.identifier.scopusid | 2-s2.0-84862292545 | - |
dc.citation.endPage | 724 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 719 | - |
dc.citation.title | JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | - |
dc.citation.volume | 24 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Immunohistochemistry | - |
dc.subject.keywordAuthor | in situ hybridization | - |
dc.subject.keywordAuthor | pigs | - |
dc.subject.keywordAuthor | Porcine reproductive and respiratory syndrome virus | - |
dc.subject.keywordPlus | LELYSTAD VIRUS | - |
dc.subject.keywordPlus | UNITED-STATES | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | GENOTYPES | - |
dc.subject.keywordPlus | US | - |
dc.subject.keywordPlus | PRRSV | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | THAILAND | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | PIGS | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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