Human amniotic fluid-derived stem cells expressing cytosine deaminase and thymidine kinase inhibits the growth of breast cancer cells in cellular and xenograft mouse models
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, N-H | - |
dc.contributor.author | Hwang, K-A | - |
dc.contributor.author | Yi, B-R | - |
dc.contributor.author | Lee, H. J. | - |
dc.contributor.author | Jeung, E-B | - |
dc.contributor.author | Kim, S. U. | - |
dc.contributor.author | Choi, K-C | - |
dc.date.available | 2019-05-29T07:33:02Z | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 0929-1903 | - |
dc.identifier.issn | 1476-5500 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20269 | - |
dc.description.abstract | As human amniotic fluid-derived stem cells (hAFSCs) are capable of multiple lineage differentiation, extensive self-renewal and tumor targeting, they may be valuable for clinical anticancer therapies. In this study, we used hAFSCs as vehicles for targeted delivery of therapeutic suicide genes to breast cancer cells. hAFSCs were engineered to produce AF2.CD-TK cells in order to express two suicide genes encoding bacterial cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV-TK) that convert non-toxic prodrugs, 5-fluorocytosine (5-FC) and mono-phosphorylate ganciclovir (GCV-MP), into cytotoxic metabolites, 5-fluorouracil (5-FU) and triphosphate ganciclovir (GCV-TP), respectively. In cell viability test in vitro, AF2.CD-TK cells inhibited the growth of MDA-MB-231 human breast cancer cells in the presence of the 5-FC or GCV prodrugs, or a combination of these two reagents. When the mixture of 5-FC and GCV was treated together, an additive cytotoxic effect was observed in the cell viability. In animal experiments using female BALB/c nude mouse xenografts, which developed by injecting MDA-MB-231 cells, treatment with AF2.CD-TK cells in the presence of 5-FC and GCV significantly reduced tumor volume and weight to the same extent seen in the mice treated with 5-FU. Histopathological and fluorescent staining assays further showed that AF2.CD-TK cells were located exactly at the site of tumor formation. Furthermore, breast tissues treated with AF2.CD-TK cells and two prodrugs maintained their normal structures (for example, the epidermis and reticular layers) while breast tissue structures in 5-FU-treated mice were almost destroyed by the potent cytotoxicity of the drug. Taken together, these results indicate that AF2.CD-TK cells can serve as excellent vehicles in a novel therapeutic cell-based gene-directed prodrug system to selectively target breast malignancies. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Human amniotic fluid-derived stem cells expressing cytosine deaminase and thymidine kinase inhibits the growth of breast cancer cells in cellular and xenograft mouse models | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/cgt.2012.15 | - |
dc.identifier.bibliographicCitation | CANCER GENE THERAPY, v.19, no.6, pp 412 - 419 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000304191400006 | - |
dc.identifier.scopusid | 2-s2.0-84861198636 | - |
dc.citation.endPage | 419 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 412 | - |
dc.citation.title | CANCER GENE THERAPY | - |
dc.citation.volume | 19 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | amniotic fluid-derived stem cells | - |
dc.subject.keywordAuthor | breast cancer | - |
dc.subject.keywordAuthor | cytosine deaminase | - |
dc.subject.keywordAuthor | thymidine kinase | - |
dc.subject.keywordAuthor | tumor tropism | - |
dc.subject.keywordPlus | SUICIDE GENE-THERAPY | - |
dc.subject.keywordPlus | INTERFERON-BETA MIGRATE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | BIFIDOBACTERIUM-INFANTIS | - |
dc.subject.keywordPlus | ANTITUMOR-ACTIVITY | - |
dc.subject.keywordPlus | GANCICLOVIR | - |
dc.subject.keywordPlus | VIVO | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | MELANOMA | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.