Heterologous Expression of Polygalacturonase Genes Isolated from Galactomyces citri-aurantii IJ-1 in Pichia pastoris
DC Field | Value | Language |
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dc.contributor.author | Cho, Il Jae | - |
dc.contributor.author | Yeo, In-Cheol | - |
dc.contributor.author | Lee, Nam Keun | - |
dc.contributor.author | Jung, Suk Hee | - |
dc.contributor.author | Hahm, Young Tae | - |
dc.date.available | 2019-05-29T07:38:13Z | - |
dc.date.issued | 2012-04 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.issn | 1976-3794 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20423 | - |
dc.description.abstract | The objective of this work was to isolate the polygalacturonase genes of Galactomyces citri-aurantii IJ-1 harvested from rotten citrus peels and to heterologously express these genes in Pichia pastoris. Two polygalacturonase (PG) genes from G. citri-aurantii IJ-1 were obtained and tentatively named PG1 and PG2. The genes were cloned into pPICZ alpha C, and expressed in Pichia pastoris strain GS115 with a native signal peptide or the a-factor secretion signal peptide of Saccharomyces cerevisiae. All of the recombinant proteins were successfully secreted into the culture media and confirmed as a single band with a molecular weight of 35 to 38 kDa by SDS-PAGE. The specific enzyme activities of recombinant PG1 and PG2 purified by His-tag affinity resin were 4,749 and 6,719 U/mg, respectively, with an optimal pH and temperature of pH 4.0 and 50 degrees C. The Michaelis-Menten kinetic constants for PG1 and PG2, K-m, were confirmed to be 0.94 and 0.84 mM, respectively. In the presence of Mn2+, the activity of PG1 and PG2 were increased to 160.8 and 146.4% of normal levels, respectively. In contrast, Cu2+ and Fe3+ acted as strong inhibitors to the PGs. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.title | Heterologous Expression of Polygalacturonase Genes Isolated from Galactomyces citri-aurantii IJ-1 in Pichia pastoris | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12275-012-1290-7 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.50, no.2, pp 332 - 340 | - |
dc.identifier.kciid | ART001653556 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000303374300021 | - |
dc.identifier.scopusid | 2-s2.0-84860376542 | - |
dc.citation.endPage | 340 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 332 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 50 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | polygalacturonase | - |
dc.subject.keywordAuthor | Galactomyces citri-aurantii | - |
dc.subject.keywordAuthor | Pichia pastoris | - |
dc.subject.keywordAuthor | heterologous expression | - |
dc.subject.keywordPlus | THERMOPHILIC BACILLUS SP | - |
dc.subject.keywordPlus | THERMOSTABLE PECTATE LYASE | - |
dc.subject.keywordPlus | ASPERGILLUS-NIGER | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | ENDO-POLYGALACTURONASE | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | SP TS-47 | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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