Application of a Reassortant Cucumber mosaic virus Vector for Gene Silencing in Tomato and Chili Pepper Plants
DC Field | Value | Language |
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dc.contributor.author | Hong, Jin Sung | - |
dc.contributor.author | Rhee, Sun-Ju | - |
dc.contributor.author | Kim, Eun-Ji | - |
dc.contributor.author | Kim, Tae-Sung | - |
dc.contributor.author | Ryu, Ki Hyun | - |
dc.contributor.author | Masuta, Chikara | - |
dc.contributor.author | Lee, Gung Pyo | - |
dc.date.available | 2019-05-29T07:39:58Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.issn | 1598-2254 | - |
dc.identifier.issn | 2093-9280 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20465 | - |
dc.description.abstract | We developed a reassortant RNA virus vector derived from Cucumber mosaic virus (CMV), which has advantages of very wide host range and can efficiently induce gene silencing in a few model plants. Certain CMV isolates, however, show limited host ranges presumably because they naturally co-evolved with their own hosts. We used a reassortant comprised of two strains of CMV, V-CMV and Gn-CMV, to broaden the host range and to develop a virus vector for virus-induced gene silencing (VIGS). Gn-CMV could infect chili pepper and tomato more efficiently than V-CMV. Gn-CMV RNA1, 3 and Y-CMV RNA2-A1 vector were newly reconstructed, and the transcript mixture of RNA I and 3 genomes of Gn-CMV and RNA2 genome of Y-CMV RNA2 containing portions of the endogenous phytoene desaturase (PDS) gene (CMV2A1::PDSs) was inoculated onto chili pepper (cv. Chung-yang), tomato (cvs. Bloody butcher, Tigerella, Silvery fir tree, and Czech bush) and Nicotianu benthamiana. All the tested plants infected by the reassortant CMV vector showed typical photo-bleaching phenotypes and reduced expression levels of PDS mRNA. These results suggest that the reassortant CMV vector would be a useful tool for the rapid induction of the RNA silencing of endogenous genes in chili pepper and tomato plants. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC PLANT PATHOLOGY | - |
dc.title | Application of a Reassortant Cucumber mosaic virus Vector for Gene Silencing in Tomato and Chili Pepper Plants | - |
dc.type | Article | - |
dc.identifier.doi | 10.5423/PPJ.NT.11.2011.0220 | - |
dc.identifier.bibliographicCitation | PLANT PATHOLOGY JOURNAL, v.28, no.1, pp 81 - 86 | - |
dc.identifier.kciid | ART001639374 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000301016300011 | - |
dc.identifier.scopusid | 2-s2.0-84862666507 | - |
dc.citation.endPage | 86 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 81 | - |
dc.citation.title | PLANT PATHOLOGY JOURNAL | - |
dc.citation.volume | 28 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Cucumber mosaic virus vector | - |
dc.subject.keywordAuthor | endogenous gene | - |
dc.subject.keywordAuthor | phytoene desaturase | - |
dc.subject.keywordAuthor | RNA silencing | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | FUNCTIONAL-ANALYSIS | - |
dc.subject.keywordPlus | MOVEMENT PROTEIN | - |
dc.subject.keywordPlus | ANTISENSE RNA | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | DEFENSE | - |
dc.subject.keywordPlus | BARLEY | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | INTERFERENCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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