Biosynthesis of a cholesterol-derived brassinosteroid, 28-norcastasterone, in Arabidopsis thaliana
DC Field | Value | Language |
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dc.contributor.author | Joo, Se-Hwan | - |
dc.contributor.author | Kim, Tae-Wuk | - |
dc.contributor.author | Son, Seung-Hyun | - |
dc.contributor.author | Lee, Woo Sung | - |
dc.contributor.author | Yokota, Takao | - |
dc.contributor.author | Kim, Seong-Ki | - |
dc.date.available | 2019-05-29T07:40:24Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.issn | 0022-0957 | - |
dc.identifier.issn | 1460-2431 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20480 | - |
dc.description.abstract | A metabolic study revealed that 28-norcastasterone in Arabidopsis is synthesized from cholesterol via the late C-6 oxidation pathway. On the other hand, the early C-6 oxidation pathway was found to be interrupted because cholestanol is converted to 6-oxocholestanol, but further metabolism to 28-norcathasterone was not observed. The 6-oxoBRs were found to have been produced from the respective 6-deoxoBRs administered to the enzyme solution, thus indicating that these 6-oxoBRs are supplied from the late C-6 oxidation pathway. Heterologously expressed CYP85A1 and CYP85A2 in yeast catalysed this C-6 oxidation, with CYP85A2 being much more efficient than CYP85A1. Abnormal growth of det2 and dwf4 was restored via the application of 28-norcastasterone and closer precursors. Furthermore, det2 and dwf4 could not convert cholesterol to cholestanol and cholestanol to 6-deoxo-28-norcathasterone, respectively. It is, therefore, most likely that the same enzyme system is operant in the synthesis of both 28-norcastasterone and castasterone. In the presence of S-adenosyl-L-methionine, the cell-free enzyme extract catalysed the C-24 methylation of 28-norcastasterone to castasterone, although the conversion rates of 28-norteasterone to teasterone and 28-nortyphasterol to typhasterol were much lower; this suggests that 28-norcastasterone is the primary precursor for the generation of C-28-BRs from C-27-BRs. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Biosynthesis of a cholesterol-derived brassinosteroid, 28-norcastasterone, in Arabidopsis thaliana | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/jxb/err354 | - |
dc.identifier.bibliographicCitation | JOURNAL OF EXPERIMENTAL BOTANY, v.63, no.5, pp 1823 - 1833 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000301297800005 | - |
dc.identifier.scopusid | 2-s2.0-84863297328 | - |
dc.citation.endPage | 1833 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1823 | - |
dc.citation.title | JOURNAL OF EXPERIMENTAL BOTANY | - |
dc.citation.volume | 63 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Arabidopsis thaliana | - |
dc.subject.keywordAuthor | brassinosteroids | - |
dc.subject.keywordAuthor | C-27-BRs biosynthesis | - |
dc.subject.keywordAuthor | 28-norcastasterone | - |
dc.subject.keywordPlus | PHASEOLUS-VULGARIS | - |
dc.subject.keywordPlus | CULTURED-CELLS | - |
dc.subject.keywordPlus | MARCHANTIA-POLYMORPHA | - |
dc.subject.keywordPlus | YEAST EXPRESSION | - |
dc.subject.keywordPlus | TOMATO | - |
dc.subject.keywordPlus | CASTASTERONE | - |
dc.subject.keywordPlus | CYTOCHROME-P450 | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | BRASSINOLIDE | - |
dc.subject.keywordPlus | CYP85A2 | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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