Simultaneous analysis of six major compounds in Osterici Radix and Notopterygii Rhizoma et Radix by HPLC and discrimination of their origins from chemical fingerprint analysis
- Authors
- Kim, Sungun; Kim, Kyoung Young; Han, Chi Soo; Ki, Kim Sool; Min, Kim Ji; Zhang, Xiaodan; Whang, Wan Kyunn
- Issue Date
- Mar-2012
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Osterici Radix; Notopterygii Rhizoma et Radix; HPLC-UV; Quality evaluation; Fingerprint
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.35, no.4, pp 691 - 699
- Pages
- 9
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 35
- Number
- 4
- Start Page
- 691
- End Page
- 699
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20494
- DOI
- 10.1007/s12272-012-0413-3
- ISSN
- 0253-6269
1976-3786
- Abstract
- Osterici Radix, the dried roots of Ostericum koreanum Maximowicz, has long been used to treat cold, headache and arthralgia in Chinese medicine. Notopterygii Rhizoma et Radix, the dried rhizomes and roots of Notopterygium incisum Ting et H.T. Chang or Notopterygium forbesii Boiss (family: Umbelliferae), are well-known traditional Chinese medicines. These all herbal medicines were named kangwhoal in China, Japan and Korea. However no discrimination methods for the origin and no simultaneous analysis methods for bioactive components are currently available. We developed simultaneous analysis of six biomarkers including nodakenin (1), oxypeucedanin (2), bisabolangelone (3), notopterol (4), imperatorin (5), and isoimperatorin (6) using HPLC method. HPLC chromatographic separation was conducted with C18 column and mobile phase of acetonitrile (A) - water (B) (gradient, (A) 35%-65% (30 min)). The analytical condition was validated with specificity, selectivity, linearity (correlation coefficients of all bio marker were more than 0.999.), recovery (94.9-106.8%), precision and accuracy (intra and inter RSD < 2.99%). In addition, principal component analysis and cluster analysis was performed on analytical data of 38 commercial kangwhoal samples from different places. We confirmed to classify kangwhoal origins and this study was applied to quality evaluation and chemotaxonomy of medicinal herbs.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.