Functional Expression and Characterization of Recombinant NADPH-P450 Reductase from Malassezia globosa
DC Field | Value | Language |
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dc.contributor.author | Lee, Hwayoun | - |
dc.contributor.author | Park, Hyoung-Coo | - |
dc.contributor.author | Lim, Young-Ran | - |
dc.contributor.author | Lee, Im-Soon | - |
dc.contributor.author | Kim, Beom Joon | - |
dc.contributor.author | Seong, Cheul-Hun | - |
dc.contributor.author | Chun, Young-Jin | - |
dc.contributor.author | Kim, Donghak | - |
dc.date.available | 2019-05-29T09:07:34Z | - |
dc.date.issued | 2012-01 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20616 | - |
dc.description.abstract | Malassezia globosa is a common pathogenic fungus that causes skin diseases including dandruff and seborrheic dermatitis in humans. Analysis of its genome identified a gene (MGL_1677) coding for a putative NADPH-P450 reductase (NPR) to support the fungal cytochrome P450 enzymes. The heterologously expressed recombinant M. globosa NPR protein was purified, and its functional features were characterized. The purified protein generated a single band on SDS-PAGE at 80.74 kDa and had an absorption maximum at 452 nm, indicating its possible function as an oxidized flavin cofactor. It evidenced NADPH-dependent reducing activity for cytochrome c or nitroblue tetrazolium. Human P450 1A2 and 2A6 were able to successfully catalyze the O-deethylation of 7-ethoxyresorufin and the 7-hydroxylation of coumarin, respectively, with the support of the purified NPR. These results demonstrate that purified NPR is an orthologous reductase protein that supports cytochrome P450 enzymes in M. globosa. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Functional Expression and Characterization of Recombinant NADPH-P450 Reductase from Malassezia globosa | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.1107.07058 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.1, pp 141 - 146 | - |
dc.identifier.kciid | ART001630644 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000299716400018 | - |
dc.identifier.scopusid | 2-s2.0-84930481876 | - |
dc.citation.endPage | 146 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 141 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 22 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Reductase | - |
dc.subject.keywordAuthor | cytochrome P450 | - |
dc.subject.keywordAuthor | Malassezia globosa | - |
dc.subject.keywordAuthor | NADPH | - |
dc.subject.keywordAuthor | 7-ethoxyresorufin | - |
dc.subject.keywordAuthor | coumarin | - |
dc.subject.keywordPlus | CYTOCHROME P450 REDUCTASE | - |
dc.subject.keywordPlus | FMN | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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