The Protective Effect of Quercetin-3-O-beta -D-Glucuronopyranoside on Ethanol-induced Damage in Cultured Feline Esophageal Epithelial Cells
DC Field | Value | Language |
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dc.contributor.author | Cho, Jung Hyun | - |
dc.contributor.author | Park, Sun Young | - |
dc.contributor.author | Lee, Ho Sung | - |
dc.contributor.author | Whang, Wan Kyunn | - |
dc.contributor.author | Sohn, Uy Dong | - |
dc.date.available | 2019-05-29T09:39:35Z | - |
dc.date.issued | 2011-12 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.issn | 2093-3827 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21074 | - |
dc.description.abstract | Quercetin-3-O-beta -D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus Herba. We aimed to explore its protective effect against ethanol-induced cell damage and the mechanism involved in the effect in feline esophageal epithelial cells (EEC). Cell viability was tested and 2',7'-dichlorofluorescin diacetate assay was used to detect intracellular H2O2 production. Western blotting analysis was performed to investigate MAPK activation and interleukin 6 (IL-6) expression. Exposure of cells to 10% ethanol time-dependently decreased cell viability. Notably, exposure to ethanol for 30 min decreased cell viability to 43.4%. When cells were incubated with 50 mu M QGC for 12 h prior to and during ethanol treatment, cell viability was increased to 65%. QGC also inhibited the H2O2 production and activation of ERK 1/2 induced by ethanol. Pretreatment of cells with the NADPH oxidase inhibitor, diphenylene iodonium, also inhibited the ethanol-induced ERK 1/2 activation. Treatment of cells with ethanol for 30 or 60 min in the absence or presence of QGC exhibited no changes in the IL-6 expression or release compared to control. Taken together, the data indicate that the cytoprotective effect of QGC against ethanol-induced cell damage may involve inhibition of ROS generation and downstream activation of the ERK 1/2 in feline EEC. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.title | The Protective Effect of Quercetin-3-O-beta -D-Glucuronopyranoside on Ethanol-induced Damage in Cultured Feline Esophageal Epithelial Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.4196/kjpp.2011.15.6.319 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.15, no.6, pp 319 - 326 | - |
dc.identifier.kciid | ART001617927 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000298905800001 | - |
dc.identifier.scopusid | 2-s2.0-84862914361 | - |
dc.citation.endPage | 326 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 319 | - |
dc.citation.title | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.citation.volume | 15 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Flavonoid | - |
dc.subject.keywordAuthor | Hydrogen peroxide | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | Esophageal epithelial cell | - |
dc.subject.keywordAuthor | Ethanol | - |
dc.subject.keywordPlus | OXYGEN SPECIES PRODUCTION | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | ALCOHOL-RELATED DISEASES | - |
dc.subject.keywordPlus | REACTIVE OXYGEN | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | MAP KINASE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | INDUCED CYTOTOXICITY | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | CYTOKINE PRODUCTION | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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