Purification and characterization of a cytosolic Ca2+-independent phospholipase A(2) from bovine brain
DC Field | Value | Language |
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dc.contributor.author | Jeong, Eui Man | - |
dc.contributor.author | Ahn, Kyong Hoon | - |
dc.contributor.author | Jeon, Hyung Jin | - |
dc.contributor.author | Kim, Ha Dong | - |
dc.contributor.author | Lee, Ho Sup | - |
dc.contributor.author | Jung, Sung Yun | - |
dc.contributor.author | Jung, Kwang Mook | - |
dc.contributor.author | Kim, Seok Kyun | - |
dc.contributor.author | Bonventre, Joseph V. | - |
dc.contributor.author | Kim, Dae Kyong | - |
dc.date.available | 2019-05-29T11:32:24Z | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21151 | - |
dc.description.abstract | The Ca2+-independent phospholipase A(2) (iPLA(2)) subfamily of enzymes is associated with arachidonic acid (AA) release and the subsequent increase in fatty acid turnover. This phenomenon occurs not only during apoptosis but also during inflammation and lymphocyte proliferation. In this study, we purified and characterized a novel type of iPLA(2) from bovine brain. iPLA(2) was purified 4,174-fold from the bovine brain by a sequential process involving DEAE-cellulose anion exchange, phenyl-5PW hydrophobic interaction, heparin-Sepharose affinity, Sephacryl S-300 gel filtration, Mono S cation exchange, Mono Q anion exchange, and Superose 12 gel filtration. A single peak of iPLA(2) activity was eluted at an apparent molecular mass of 155 kDa during the final Superose 12 gel-filtration step. The purified enzyme had an isoelectric point of 5.3 on twodimensional gel electrophoresis (2-DE) and was inhibited by arachidonyl trifluoromethyl ketone (AACOCF(3)), Triton X-100, iron, and Ca2+. However, it was not inhibited by bromoenol lactone (BEL), an inhibitor of iPLA(2), and adenosine triphosphate (ATP). The spot with the iPLA(2) activity did not match with any known protein sequence, as determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. Altogether, these data suggest that the purified enzyme is a novel form of cytosolic iPLA(2). | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Purification and characterization of a cytosolic Ca2+-independent phospholipase A(2) from bovine brain | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s10059-011-1058-7 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.32, no.5, pp 405 - 413 | - |
dc.identifier.kciid | ART001602703 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000297627900002 | - |
dc.identifier.scopusid | 2-s2.0-84855650333 | - |
dc.citation.endPage | 413 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 405 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 32 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | brain | - |
dc.subject.keywordAuthor | Ca2+-independent PLA(2) | - |
dc.subject.keywordAuthor | characterization | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordPlus | CALCIUM-INDEPENDENT PHOSPHOLIPASE | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | MICE DEFICIENT | - |
dc.subject.keywordPlus | PROSTAGLANDIN SYNTHESIS | - |
dc.subject.keywordPlus | TRIFLUOROMETHYL KETONE | - |
dc.subject.keywordPlus | DOCOSAHEXAENOIC ACID | - |
dc.subject.keywordPlus | RAT-BRAIN | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | SCHIZOPHRENIA | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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