The Mechanism of MAP Kinase Activation under Acidic Condition in Feline Esophageal Smooth Muscle Cells
DC Field | Value | Language |
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dc.contributor.author | Park, Sun Young | - |
dc.contributor.author | Lee, Young Ju | - |
dc.contributor.author | Min, Youngsil | - |
dc.contributor.author | Kim, Hak Rim | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Sohn, Uy Dong | - |
dc.date.available | 2019-05-29T11:35:05Z | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 0253-6269 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21236 | - |
dc.description.abstract | Reflux esophagitis results from repeated exposure of the esophagus to acidic gastric juice or bile-containing duodenal contents. In Barrett's adenocarcinoma, acid increases proliferation via ERK and p38 MAPK activation. This study was focused on determination of the mechanism(s) underlying MAPKs (ERK 1/2, p38 MAPK, and JNK) activation induced by acidic medium at pH 4 in normal feline primary cultured esophageal smooth muscle cells (FESMCs). We detected ERK 1/2 and p38 MAPK phosphorylation after exposure to pH 4 or neutral media in the presence or absence of several inhibitors and quantified the MAPK levels using western blotting analysis and densitometry. Acidic medium markedly increased the phosphorylation of ERK 1/2 and p38 MAPK within 10 min. Acid-induced ERK 1/2 and p38 MAPK activation was inhibited by pertussis toxin (PTX-sensitive G(i/o) protein inhibitor), DEDA (phospholipase (PL) A(2) inhibitor), pCMB (PLD inhibitor), GF109203X (protein kinase C (PKC) inhibitor) and D609 (phosphatidylcholine-specific PLC inhibitor). But, genistein (tyrosine kinase inhibitor), forskolin (adenylate cyclase activator) and U73122 (phosphatidylinositol-specific PLC inhibitor) had no effect on acid-induced ERK1/2 and p38 MAPK activation. These findings indicate that the activation of ERK 1/2 and p38 MAPK pathways by acidic conditions, at least in part, may be mediated by activation of the G(i/o) protein coupled receptors, PC-PLC, PLD, PLA(2), and PKC in FESMCs. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PHARMACEUTICAL SOC KOREA | - |
dc.title | The Mechanism of MAP Kinase Activation under Acidic Condition in Feline Esophageal Smooth Muscle Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12272-011-1020-4 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF PHARMACAL RESEARCH, v.34, no.10, pp 1759 - 1768 | - |
dc.identifier.kciid | ART001597136 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000296800200022 | - |
dc.identifier.scopusid | 2-s2.0-82055164294 | - |
dc.citation.endPage | 1768 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1759 | - |
dc.citation.title | ARCHIVES OF PHARMACAL RESEARCH | - |
dc.citation.volume | 34 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Esophageal epithelial cell | - |
dc.subject.keywordAuthor | Acid | - |
dc.subject.keywordAuthor | ERK 1/2 | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.subject.keywordAuthor | Reflux esophagitis | - |
dc.subject.keywordAuthor | Signal transduction | - |
dc.subject.keywordPlus | PROTEIN-COUPLED RECEPTORS | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION PATHWAYS | - |
dc.subject.keywordPlus | ADENOCARCINOMA CELLS | - |
dc.subject.keywordPlus | BARRETTS-ESOPHAGUS | - |
dc.subject.keywordPlus | CAT ESOPHAGEAL | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 EXPRESSION | - |
dc.subject.keywordPlus | CIRCULAR MUSCLE | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | P38 | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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