WNT/beta-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum
DC Field | Value | Language |
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dc.contributor.author | Lee, Haeyong | - |
dc.contributor.author | Bae, Sungmin | - |
dc.contributor.author | Kim, Yeong Shik | - |
dc.contributor.author | Yoon, Yoosik | - |
dc.date.available | 2019-05-29T11:35:54Z | - |
dc.date.issued | 2011-09 | - |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.issn | 1879-0631 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21265 | - |
dc.description.abstract | Aims: This study was conducted to suggest the role of WNT/beta-catenin pathway in the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum. Main methods: Gene knockdown experiments using small interfering RNA (siRNA) transfection were conducted to elucidate crucial role of beta-catenin in the anti-adipogenic effects of platycodin D. Real-Time PCR and Western blot were used to analyze the expression levels of mRNAs and proteins in the WNT/beta-catenin pathway. Key findings: During the adipocyte differentiation of 313-L1 cells, members of the WNT/beta-catenin pathway were normally down-regulated, whereas platycodin D significantly reinstated the WNT/beta-catenin pathway. The mRNA and protein expressions of disheveled (DVL) 2, which stabilize beta-catenin, were increased by platycodin D treatment, but the protein level of AXIN, which induces the degradation of beta-catenin, was decreased in platycodin D-treated cells. The nuclear level of beta-catenin was normally down-regulated during adipogenesis, but platycodin D treatment led to the accumulation of beta-catenin in the nucleus which resulted in the up-regulation of its target genes, cyclin D (CCND) 1 and peroxisome proliferator-activated receptor gamma (PPAR)gamma. The anti-adipogenic effects of platycodin D were significantly attenuated in beta-catenin siRNA-transfected cells compared with those of control siRNA-transfected cells. beta-catenin siRNA transfection significantly recovered the levels of PPAR gamma. CCAAT/enhancer binding protein (C/EBP)alpha and fatty acid binding protein (FABP)4 as well as intracellular lipid droplet formation, all of which were reduced by platycodin D treatment. Significance: WNT/beta-catenin pathway can be used as a therapeutic target of natural compounds for the regulation of adipogenesis. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | WNT/beta-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.lfs.2011.07.006 | - |
dc.identifier.bibliographicCitation | LIFE SCIENCES, v.89, no.11-12, pp 388 - 394 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000294397800006 | - |
dc.identifier.scopusid | 2-s2.0-80052029040 | - |
dc.citation.endPage | 394 | - |
dc.citation.number | 11-12 | - |
dc.citation.startPage | 388 | - |
dc.citation.title | LIFE SCIENCES | - |
dc.citation.volume | 89 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Adipogenesis | - |
dc.subject.keywordAuthor | 3T3-L1 | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Therapeutic compounds | - |
dc.subject.keywordAuthor | Adipogenic molecular biomarkers | - |
dc.subject.keywordPlus | ACTIVATED-RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL REGULATION | - |
dc.subject.keywordPlus | FUNCTIONAL INTERACTION | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | 3T3-L1 ADIPOCYTE | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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