Ultrasonic-assisted Micellar Extraction and Cloud-point Pre-concentration of Major Saikosaponins in Radix Bupleuri using High Performance Liquid Chromatography with Evaporative Light Scattering Detection
DC Field | Value | Language |
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dc.contributor.author | Suh, Joon Hyuk | - |
dc.contributor.author | Yang, Dong-Hyug | - |
dc.contributor.author | Han, Sang Beom | - |
dc.date.available | 2019-05-29T11:37:40Z | - |
dc.date.issued | 2011-08-20 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.issn | 1229-5949 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21334 | - |
dc.description.abstract | A new ultrasonic-assisted micellar extraction and cloud-point pre-concentration method was developed for the determination of major saikosaponins, namely saikosaponins -A, -C and -D, in Radix Bupleuri by high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD). The non-ionic surfactant Genapol X-080 (oligoethylene glycol monoalkyl ether) was chosen as the extraction additive and parameters affecting the extraction efficiency were optimized. The highest yield was obtained with 10% (w/v) Genapol X-080, a liquid/solid ratio of 200:1 (mL/g) and ultrasonic-assisted extraction for 40 min. In addition, the optimum cloud-point pre-concentration was reached with 10% sodium sulfate and equilibration at 60 degrees C for 30 min. Separation was achieved on an Ascentis Express C18 column (100 x 4.6 mm i.d., 2.7 mu m) using a binary mobile phase composed of 0.1% acetic acid and acetonitrile. Saikosaponins were detected by ELSD, which was operated at a 50 degrees C drift tube temperature and 3.0 bar nebulizer gas (N(2)) pressure. The water-based solvent modified with Genapol X-080 showed better extraction efficiency compared to that of the conventional solvent methanol. Recovery of saikosaponins ranged from 93.1 to 101.9%. An environmentally-friendly extraction method was successfully applied to extract and enrich major saikosaponins in Radix Bupleuri. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.title | Ultrasonic-assisted Micellar Extraction and Cloud-point Pre-concentration of Major Saikosaponins in Radix Bupleuri using High Performance Liquid Chromatography with Evaporative Light Scattering Detection | - |
dc.type | Article | - |
dc.identifier.doi | 10.5012/bkcs.2011.32.8.2637 | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.32, no.8, pp 2637 - 2642 | - |
dc.identifier.kciid | ART001576589 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000297383900022 | - |
dc.identifier.scopusid | 2-s2.0-80051983403 | - |
dc.citation.endPage | 2642 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2637 | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 32 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Saikosaponin | - |
dc.subject.keywordAuthor | Cloud-point extraction | - |
dc.subject.keywordAuthor | Genapol X-080 | - |
dc.subject.keywordAuthor | Radix Bupleuri | - |
dc.subject.keywordAuthor | HPLC-ELSD | - |
dc.subject.keywordPlus | MEDIATED PHASE SEPARATIONS | - |
dc.subject.keywordPlus | NONIONIC SURFACTANT | - |
dc.subject.keywordPlus | GENUS BUPLEURUM | - |
dc.subject.keywordPlus | TRITON X-100 | - |
dc.subject.keywordPlus | REMOVAL | - |
dc.subject.keywordPlus | HPLC | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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