Molecular Characterization and Mitogenic Activity of a Lectin from Purse Crab Philyra Pisum
- Authors
- Na, Jong Cheon; Park, Byung Tae; Chung, Woo Hyuk; Kim, Ha Hyung
- Issue Date
- Aug-2011
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Crab; Lectin; Molecular characterization; Mitogenic activity; Lymphocyte; Splenocyte
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.15, no.4, pp 241 - 244
- Pages
- 4
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 15
- Number
- 4
- Start Page
- 241
- End Page
- 244
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21394
- DOI
- 10.4196/kjpp.2011.15.4.241
- ISSN
- 1226-4512
2093-3827
- Abstract
- A lectin from the hemolymph of purse crab, Philyra pisum, was found to have anti-proliferative activity on human lung cancer cells by our laboratory. In this study, P. pisum lectin (PPL) was molecularly characterized including molecular mass, amino acid sequences, amino acid composition, and the effects of metal ions, temperature, and pH on the activity. We found that PPL showed mitogenic activity on human lymphocytes and BALB/c mouse splenocytes. The mitogenic activity (maximum stimulation index, SI=9.57 +/- 0.59) of PPL on human lymphocytes was higher than that of a standard well-known plant mitogen, concanavalin A (maximum SI=8.80 +/- 0.59). The mitogenic activity mediated by PPL is required for optimum dosing, and higher or lower concentrations caused decreases in mitogenic response. PPL also induced mitogenic activity on mouse splenocytes, however, the maximum SI (1.77 +/- 0.09) on mouse splenocytes of PPL was lower than that (2.14 +/- 0.15) of concanavalin A. In conclusion, PPL is a metal ion-dependent monomer lectin with mitogenic activity, and could be used as a lymphocyte or splenocyte stimulator.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.