Hemocyanin-derived phenoloxidase activity with broad temperature stability extending into the cold environment in hemocytes of the hair crab Erimacrus isenbeckii
- Authors
- Kim, Sang Gu; Jung, Byung Wook; Kim, HaHyung
- Issue Date
- Jun-2011
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Hair crab; Erimacrus isenbeckii; Hemocyte lysate supernatant; Phenoloxidase activity; Hemocyanin
- Citation
- COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v.159, no.2, pp 103 - 108
- Pages
- 6
- Journal Title
- COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
- Volume
- 159
- Number
- 2
- Start Page
- 103
- End Page
- 108
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21477
- DOI
- 10.1016/j.cbpb.2011.02.004
- ISSN
- 1096-4959
1879-1107
- Abstract
- Phenoloxidase (PO) activity is a major component of the innate immune response in arthropods. In this study, we characterized PO activity from the hair crab Erimacrus isenbeckii, which inhabits very cold regions (2.4-3.4 degrees C) of the Bering Sea. Hemocyte lysate supernatant (HLS) prepared from E. isenbeckii was inactive HLS until activated by nonspecific agents such as sodium dodecyl sulfate and trypsin, and elicitors such as lipopolysaccharide and lipoteichoic acid from the cell wall constituent of bacteria. The PO activity was maximal at 4 degrees C, decreased slightly at temperatures up to 60 degrees C, and fell rapidly at 80 degrees C. Both L-DOPA and catechol were efficient substrates for the PO (EC 1.10.3.1), with K(m) values of 0.96 and 1.15 mM, respectively, whereas tyrosine and hydroquinone were not. We isolated a protein fraction from HLS as a hexamer of 75 kDa units with 216.7-fold higher PO activity than that of the HLS. The N-terminal amino acid analysis of an isolated protein revealed 80% sequence identity to hemocyanins from other crabs. These results suggest that cold-adapted hemocyanin-derived PO activity is important to the survival of these crabs. This is the first report of a crab PO activity with broad temperature stability extending into the cold environment (C) 2011 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21477)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.