Dendritic Cells stimulated with outer membrane protein A (OmpA) of Salmonella typhimurium generate effective anti-tumor immunity
DC Field | Value | Language |
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dc.contributor.author | Jang, Min Ja | - |
dc.contributor.author | Kim, Jee-Eun | - |
dc.contributor.author | Chung, Yoon Hee | - |
dc.contributor.author | Lee, Won Bok | - |
dc.contributor.author | Shin, Yong Kyoo | - |
dc.contributor.author | Lee, Jun Sik | - |
dc.contributor.author | Kim, Daejin | - |
dc.contributor.author | Park, Yeong-Min | - |
dc.date.available | 2019-05-29T13:38:25Z | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 0264-410X | - |
dc.identifier.issn | 1873-2518 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21670 | - |
dc.description.abstract | Gram-negative bacterial outer membrane proteins (Omps) have an important role in pathogenesis and signal reception. We previously reported that Acinetobacter OmpA (AbOmpA) induced maturation of bone marrow-derived dendritic cells (BMDCs) and that AbOmpA-primed DCs produced IL-12 which generated Th1 CD4(+) T-cells. We analyzed the effects of Salmonella typhimurium OmpA (OmpA-Sal) on dendritic cell (DC) maturation in the present study, and determined that tumor antigen-pulsed DCs stimulated with OmpA-Sal induced anti-tumor responses in a mouse model. OmpA-Sal activated BMDCs by augmenting expression of MHC class II and of the co-stimulatory molecules CD80 and CD86. RT-PCR revealed that IL-12(p40) gene expression is highly augmented in OmpA-Sal-stimulated BMDCs. DNA (CRT/E7) vaccination combined with OmpA-Sal stimulation generated more antigen-specific CD8(+) T-cells in the present study. Certain antigen-pulsed BMDCs stimulated with OmpA-Sal induced strong PADRE-specific CD4(+) and E7-specific CDS+ T-cell responses. In addition, BMDCs stimulated with OmpA-Sal (OmpA-Sal-BMDCs) and pulsed with both E7 and PADRE peptide generated greater numbers of E7-specific CDS+ effector and memory T-cells than those pulsed with E7 peptide alone. E7- and PADRE-expressing OmpA-Sal-BMDC vaccines resulted in significant long-term protective anti-tumor effects in vaccinated mice. Our data suggested that E7- and PADRE-expressing BMDCs that were matured in the presence of OmpA-Sal might enhance anti-tumor immunity and support the therapeutic use of OmpA-Sal in DC-based immunotherapy. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Dendritic Cells stimulated with outer membrane protein A (OmpA) of Salmonella typhimurium generate effective anti-tumor immunity | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.vaccine.2011.01.036 | - |
dc.identifier.bibliographicCitation | VACCINE, v.29, no.13, pp 2400 - 2410 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000289140500009 | - |
dc.identifier.scopusid | 2-s2.0-79952362367 | - |
dc.citation.endPage | 2410 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 2400 | - |
dc.citation.title | VACCINE | - |
dc.citation.volume | 29 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Dendritic cells | - |
dc.subject.keywordAuthor | Outer membrane protein A | - |
dc.subject.keywordAuthor | Anti-tumor immunity | - |
dc.subject.keywordPlus | TOLL-LIKE RECEPTORS | - |
dc.subject.keywordPlus | CD4(+) T-CELLS | - |
dc.subject.keywordPlus | DNA VACCINE POTENCY | - |
dc.subject.keywordPlus | INNATE IMMUNITY | - |
dc.subject.keywordPlus | LANGERHANS CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | MELANOMA | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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